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Determination of Rhein,Emodin,Chrysophanol,Physcion and Aloe-emodin in JinYiNiaoShi Granules and Radix Rhei by HPLC

Cao Hong and Liu Yun(Institute for Drug Control of PLA,Beijing,100071)  
HPLC was used to determine the contents of rhein,emodin,chrysophanol,physcion and aloe-emodin in JinYiNiaoShi granules and rhubarb radix.The HPLC system consisted of Alltima C18 column(250mm×46mm,5μm),methanol—01% phosphoric acid(90∶10)mixture as mobile phase,with detection at 440nm,flow rate 10mL/min and column temperature at 30℃.For JinYiNiaoShi granules,the mean recovery of 981%(RSD=19%)for rhein,978%(RSD=29%)for emodin,979%(RSD=24%)for chrysophanol,970%(RSD=10)for physcion and 1009%(RSD=23%)for aloe-emodin is obtained,respectively.The method is sensitive,rapid and accurate.
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