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《Chinese Remedies & Clinics》 2003-01
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Expression of HLA-DM and-DR gene in rheumatoid arthritis patients in the Han nationality

LIU Xiang yuan ,ZHU Ji ping,MIN Wei qi,HUANG Jian sheng. *Department of Rheumatology,PLA General Hospital,Beijing 100853,China  
Objective To investigate the expression of HLA DM (DMA and DMB) gene in patients with rheumatoid arthritis (RA) in the Han nationality.Methods HLA DMA, DMB and DRB transcripts in purified peripheral blood B cells with anti CD19 mAb coated magnetic beads from 36 RA patients,26 ankylosing spondylitis (AS) and 33 matched normal control subjects were analyzed by semiquantitative and competitive RT PCR.Meanwhile,the expression of HLA DM and DR proteins was also quantified by Western blot.Results Compared with normal controls or AS patients,only the transcription of HLA DMA and DMB from RA patients was significantly decreased ( P 0 01),but no difference in HLA DRB ( P 0 05).An mean DM∶DR ratio in RA patients was significantly lower than that in normal controls ( P 0 01).The mean ratio of DMA/β actin in RA patients was dramatically decreased compared with that in normal controls at protein level ( P 0 01),but DRA/β actin showed no significant difference between the two groups ( P 0 05).Conclusion This specific HLA DM down regulation might play an important role in the processes of antigen presentation and autoimmune in RA.
【Fund】: 国家自然科学基金资助项目 (39970 699)
【CateGory Index】: R593.22
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1 Department of Clinical Microbiology and Immunology, Guiyang Medical College(Guiyang 550004) Jiang Hongmei Wang Yongxia;The linkage analysis of HLA-DRB_1 and polymorphisms of HLA-DM gene[J];Shaanxi Medical Journal;2008-09
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1 YANG Wen-lin1, YANG Jian1, CHEN Sheng-qiang2, XIE Fang-ying1, HUANG Xin-yu1, YE Ting1 1Department of Dermatology, the Second Affiliated Hospital, 2Institute of Neurology, Guangzhou Medical College, Guangzhou 510260, China;HLA-DM gene polymorphism in Cantonese with condyloma acuminata[J];Journal of Southern Medical University;2006-07
2 MIN Weiqi,NIU Yaowen,WANG Cuiru,et al.First Department of Internal Medicine,No.466 Hospital of PLA,Beijing 100089,China;Relationship between polymorphism in low molecular weight polypeptides-2 gene and rheumatoid arthritis in Han nationality[J];Chinese Journal of Rheumatology;2001-01
3 LU Wei 1, LI Xiang pei 2, SHI Xiao ming 1, XU Yuan yong 1, HU Yi song 1, YANG Shi gui 1, YE Dong qing 1. 1.Department of Epidemiology, Anhui Medical University, Hefei 230032, China; 2.Department of Rheumatism, Anhui Province Hospital, Hefei;The relationship of HLA-DM genetic polymorphisms with systemic lupus erythematosus in Chinese[J];Chinese Journal of Disease Control and Prevention;2002-02
4 LIUXiang yuan, MINWei qi, Huang Feng,Li Sheng guang(Department of Rheumatology, PLA General Hospital , Beijing 100853,China);Study on HLA-DM polymorphisms in patients with rheumatoid arthritis in Chinese population[J];Academic Journal of Pla Postgraduate Medical School;2002-01
5 NI Bing (Institute of Immunology, PLA, Third Military Medical University, Chon gqing 400038, China);Proteasome and processing and presentation of MHC-Ⅰ restricted antigen[J];Immunological Journal;2001-05
6 ZHANG Yong,ZHU Xiao-chun,SUN Xue-qing(Medical College of Shanghai Jiaotong University,Shanghai Institute of Immunology,Shanghai 200025,China);Polymorphism of TAP,LMP and HLA-DM genes of Chinese Han population in Shanghai area[J];Current Immunology;2006-01
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8 Xu Qunqing Wei Yesheng Liang Liyan Scientific Laboratory Center , Youjiang Medical College for Nationalities (Baise 533000);Study of CD14 gene promoter region C-260T polymorphism in the Hans in Guangxi province[J];Shaanxi Medical Journal;2007-11
9 SANG Yan mei, YAN Chun, ZHU Cheng, NI Gui chen, HU Ya mei. Department of Beijing Children′s Hospital Affiliated to Capital University of Medical Sciences, Beijng 100045, China;Association of human leukocyte antigen non-classical genes with type 1 diabetes[J];Chinese Journal of Pediatrics;2003-04
10 WE Fei, HUANG Han-tin , HUANG Zhen-lie, LI Lai-yu , LIANG Li-yan , CHEN Xue-min . Department of Environmental Hygiene, Tongji medical College , Huazhong University of Science and Technology , Wuhan430030, China;Relationship of trichloroethylene-induced medicamentosa Uke dermatitis to HLA-DMA and HLA-DMB[J];Chinese Journal of Industrial Hygiene and Occupational Diseases;2006-05
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