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Preparation of Freeze-Dry Mice Nerve Growth Factor on A Medium Scale

Ren Wanqiong ,Feng Jun, Liu Baoshan,Song Guoming,Zhou Wenli (Biological and Medical Products Research Institue of Jinan Military Area,Zhumadian 463000)  
To medium scale mice nerve growth factor,mice submaxillary glandwas ground, extracted,centrifugated and defatted successively,then,the mixture was purified by CM cellulose, ultrafiltration( M r 10 000), CM sepharose Thus 2 5S Nerve Growth Factor(NGF)was obtained The yield of this product was 110mg~130mg from 1000 male mice The M r was 13000±2000 and the isoelectric point was 8 6~9 3 The result showed that puring and specific activity of NGF is higher with this method,and the production process is shorter
【CateGory Index】: R915
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