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《Pharmaceutical Biotechnology》 2007-02
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Study on Purification of Vancomycin Fermentation Broth with Macroporous Adsorbent Resin

ZHANG Yi1,DU Lian-xiang1,MA Shu-zhang1,ZHANG Ying2(1.Tianjin Key Lab of Industrial Microbiology,School of Biotechnology,Tianjin University of Science and Technology,Tianjin 300457 China;2. Tianjin Soil Fertilizer Workstation,Tianjin 300061,China)  
To study the process condition of the purification of vancomycin fermentation broth,the characteristics of static adsorption and desorption of six kinds of macroporous adsorbent resin to vancomycin were studied.The most suitable resin was selected to carry out the dynamic adsorption experiment.The concentration,pH value,flow rate of the infusion on the adsorption of the resin and the elution process were also studied.The XAD-16 resin was selected to carry out the dynamic adsorption experiment.The suitable concentration,pH value,and the flow rate of the infusion were 2.09mg/ml,9.0 and 2.5ml/min respectively.Using 10% ethanol/0.1% hydrochloric acid/water as elution solvent,vancomycin adsorbed on the resin was substantially eluted at a flow rate of 1.5ml/min,and after the adsorption and desorption of XAD-16 resin to vancomycin fermentation broth,the HPLC peak area percentage of vancomycin in the eluant could reach 85.61%.
【CateGory Index】: R944
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Chinese Journal Full-text Database 4 Hits
1 Duan Jinyou, Fang Jinian * (Shanghai Institute of Materia Medica,Chinese Academy of Sciences,Shanghai 200031);The Applications of Reversed Micellar Extraction in Separation of Biomolecules and Related Fields[J];Chinese Journal of Analytieal Chemistry;2002-03
2 GUO Li\|An 1 \ ZHU Bao\|Quan\+2\ CHEN Dai\|Jie\+2 (Chemical Department,The Fourth Military Medical University,Xi'an 710032,China) (Shanghai Institute of Pharmaceutical Industry, Shanghai 200043,China);Purification of Vancomycin by Using Magnetic Affinity Technique[J];Chinese Journal of Biotechnology;2001-05
3 ZHU Li-xian,JIN Zheng-yu (School of Food Science and Technology, Southern Yangtze University, Wuxi 214036, China);The Study on Adsorption Performance of Macroporous Resins for Resveratrol in Polygonum cuspidatum Sieb. et Zucc[J];Food Science;2005-03
4 Chen Dai-jie1,Li Ji an2,Zou Yun hua2,Liu Yao2,Ge Mei1, Jin Fei yan3,Su Xu xia1,Xu Bing xiang4 and Jin Yi ping4 (1 Shanghai Health-Creation Center for Biopharmaceutical R &D,Shanghai 201203; 2 Shanghai Institute of Pharmaceutical Industry,Shanghai 200040; 3 China Pharmaceutical University,Nanjing 210009; 4 Zhejiang Medicine Co.,Ltd.Xinchang Pharmaceutical Factory,Xinchang 312500);Process development of vancomycin[J];Chinese Journal of Antibiotics;2004-01
Chinese Journal Full-text Database 10 Hits
1 HUANG Fang-zhi,SHEN Yu-hua,XIE An-jian,QIU Ling-guang,LIU Hui(School of Chemistry and Chemical Engineering,Anhui University,Hefei 230039,China);Effect on microstructure of tryptophane extracted by reversed microemulsion[J];Journal of Anhui University(Natural Sciences);2007-02
2 ZHU Yan-yan,WANG Yao-yao,LIU Yun-qing,LIU song,CAI Chao-jing (New Drug Research and Developmental Center,North China Pharmaceulical Co.,Shijiazhuang 050015,China);Effects of Trace Elements and Precursor on the Biosynthesis of N-demethylvancomycin[J];Journal of Hebei University(Natural Science Edition);2006-05
3 LU Nan(Department of Pharmaceutical Engineering,Hebei Chemical and Pharmaceutical Vocational Technology College,Shijiazhuang Hebei 050031,China);Biological and medical application of double water phases extraction technique[J];Hebei Journal of Industrial Science and Technology;2006-03
4 LI Biao-wen, REN Zhuo, SONG Ru, WANG Rong-xue (NCPC Huasheng Co. , NCPC New Drug Centre, Shijiazhuang 050011,China);Spectrophotometric Determination of Vancomycin[J];Hebei Chemical Engineering and Industry;2006-04
5 YAN Jing,CHENG Ji-ping,DAI Li-min,ZHAI Yan-rui,ZHANG Li-li(New Drug Research and Developmental Center,North China Pharmaceulical Co.,Ltd,Shijiazhuang 050015,China);Breeding on N-demethylvancomycin High-producing Strain[J];Hebei Chemical Engineering and Industry;2007-05
6 WANG Qin1,SONG Ru2,REN Zuo1 ,WANG Rong-xue2 (1.NCPC Jintan Co.,Ltd,Shijiazhuang 050011,China;2.NCPC Huasheng Co.,Ltd,Shijiazhuang 052160,China);Application of Memtrane in Vancomycin Process[J];Hebei Chemical Engineering and Industry;2007-11
7 YANG Shan-sheng,LU Wen-cong,BAO Bo-rong (Department of Chemistry ,College of Science,Shanghai University ,Shanghai 200436,China);Progress in aqueous two-phase extraction technique and its application[J];Chemical Engineer;2004-04
8 LIU Chuan-fang,CUI Yong(Department of Chemistry and Biology Wanxi University,Anhui LU An 237012,China);Micro-micelle Extraction of p-Dihydroxybenzene in Dual-aquatic Phase[J];Chemical World;2008-07
9 ZHOU Fu-rong,SHI Hua-dong(School of Chemistry and environmental engineering,Jianghan University,Wuhan 430056,China);Study on extraction of soybean protein in saponified P_(204) microemulsion system[J];Chemical Research and Application;2007-04
10 FAN Ting-ting, WANG Xin, LIU Ying(College of Food Engineering,Harbin University of Commerce,Harbin 150076,China);Research Progress on Preparation of Resveratrol[J];Fine and Specialty Chemicals;2007-16
China Proceedings of conference Full-text Database 1 Hits
1 Wang Ji-gang,Chen Shen-wen,Yu Zi-niu (State Key Laboratory ofAgricultural Microbiology, National Engineering Research Center for Microbial Pesticides, College of Life science and Technology ,Huazhong Agricultural University, Wuhan 430070, China);Effects of Vegetable Oils on Fermentation of GA3 Produced by Gibberella jujikuroi[A];[C];2004
Chinese Journal Full-text Database 10 Hits
1 LIU Jie-feng et al(College of Chemistry and Life Science,Maoming University,Maoming,Guangdong 525000);Isolation of Endophyte and Screening for Antagonistic Bacteria in Solanaceae[J];Journal of Anhui Agricultural Sciences;2009-03
2 LUO Ming1,LU Yun2,ZHANG Xiang-lin3,CHENG Zhong-jian1 (1 College of Agronomy,Xinjiang Agricultural University,Urumqi 830052,China;2 College of Science,Xinjiang Education Institute,Urumqi 830043,China;3 Xinjiang Entry & Exit Inspection and Quaratine Bureau of the People's Repubic of China,Urumqi 830063,China);Colonization and Conduction Dynamics of Endophytic Antagonistic Bacteria in Hami Melon Plants and Their Effects on Plant Growth-promoting[J];Acta Botanica Boreali-Occidentalia Sinica;2007-04
3 HE Hong1,2, CAI Xueqing1, CHEN Yusen1, SHEN Zhaochang1, GUAN Xiong1, HU Fangping1(1.College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China; 2.Agricultural College of Zhangjiang Ocean University, Zhangjiang, Guandong 524088, China);Biological control of banana anthracnose with endophytic Bacillus subtilis BS-2 and BS-1 isolated from capsicum[J];Journal of Fujian Agricultural University(Natural Science);2002-04
4 Han Ping~1, Liu Xili1 , Liu Pengfei~1, Si Naiguo~2 ~1(Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094) ~2(China Shenyang Research Institute of Chemical Industry, Shenyang 110021);Effect of Novel Fungicide 5-(4-Chloro phenyl)-2,3-dimethyl-3- (pyridine-3)-oxazoline on Ergosterol Biosynthesis in Botrytis Cinerea by High Performance Liquid Chromatography[J];Chinese Journal of Analytical Chemistry;2006-10
5 TIAN Xing-shan 1, JIANG Xue-bing 2, SHEN Hui-fang 2, XIAN Hai-hui 2, XIE Shuang-da 2 (1. Agro-Biotech Research Institute, Guangdong Academy of Agricultural Sciences,Guangzhou 510640, China; 2. Plant Protection Research Institu;ANALYSIS OF AGRI-ANTIBIOTICS 2507 WITH HIGH PERFORMANCE LIQUID CHROMATOGRAPHY[J];Journal of South China Normal University(Natural Science);2001-04
6 XIA Zhengjun, GU Benkang, WU Aiming, and FU Zhengqing (Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014);Isoenzymatic Activities Involved in Induced Resistance of Cotton to Verticillium dahliae by Endophytic Bacteria[J];JIANGSU JOURNAL OF AGRICULTURAL SCIENCES;1997-02
7 XU Jing, XUN Guang xin, ZHAO Tong hai, ZHANG Qing wen, CHEO Ming tang (Department of Entomology, China Agricultural University, Beijing 100094);In vitro and planta bioassays of engineered endophytic bacteria in cotton with cotton bollworms[J];ACTA ENTOMOLOGICA SINICA;2000-S1
8 Gu Juefen; Cheng Gang(Division of antibiotie, China Pharmaceutical University, Nanjing 210009);Studies Isolation and Purification of Antibiotic LY-92 by Macroreticular Adsorbent H-103[J];ION EXCHANGE AND ADSORPTION;1994-06
9 Qi Gaofu\ Yang Bin\ Ye Jianren(College of Nanjing Forest Resources and Environment Nanjing Forestry University\ Nanjing 210037);Research Advances on the Toxin of the Plant Pathogenic Fungus[J];JOURNAL OF NANJING FORESTRY UNVERSITY;2000-02
10 Zhao Xiuyun\ Han Sufen(College of Forest Resources and Environment Nanjing Forestry University\ Nanjing\ 210037);Isolation, Selection and Identification of Nitrogen Fixing Bacteria in Poplar Rhizosphere[J];JOURNAL OF NANJING FORESTRY UNVERSITY;2000-03
【Secondary Citations】
Chinese Journal Full-text Database 9 Hits
1 LIU Yuan-Kun, GUO Li-An, LIANG Guo-Dong and ZHUANG Wen-Xu Central Laboratory, Tangdu Hospital Department of Biology;Preparing magnetite albumin microspheres containing adriamycin[J];Journal of The Fourth Military Medical University;1991-05
2 GUO Li-An;ZHU Bao-Quan(The Centre Laboratory, Fourth Military MedicalUniversity, Xi'an 710033, China,) ZCHEN Dai--Jie( Shanghai Institute of Pharmaceutical Industry, China);Purification of recombinant human interferon-α by magnetic affinity microsphere[J];JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY;1999-01
3 CHU Ying; JI Jian-Ye; MA Zhan-Fang; YU Yan-Chun; WU Zi-Sheng(Department of Chemistry, Northeast Normal University, Changchun, 130024;Department of Chemistry, Tonghua Noumal College,Tonghua,130000);Purification of Yeast-Lipase by the Phase Transfer Method of Reverse Micelles[J];CHEMICAL RESEARCH IN CHINESE UNIVERSITIES;1997-10
4 ZHOU Shi -Qi( Department of Chemistry, East China University of Science and Technology, Shanghai, 200237 )GUO Si - Yuan(Research Institute of Light Industry and Chemical Engineering, South China University of Technology, Guangzhou, 510641);Molecular Thermodynamic Model for Reverse Micelles System and Protein Extraction I . Molecular Thermodynamic Model for Reverse Micelles System[J];Acta Chimica Sinica;1999-05
5 HAN Jinyu LI Haijing LI Yan WANG Hua CHANG Heying Tianjin University, Tianjin 300072;STUDY ON ADSORPTION OF GINKGOLIDES AND BILOBALIDE WITH MACROPOROUS RESINS[J];Ion Exchange and Adsorption;2000-05
6 Yuan Huaibo Zhao Guohua Li Hongjun Chen Zongdao(College of Food Science,Southwest Agricultural University, Beipei, 400716);Purification of Pueraria Isoflavones with Macroreticular Resin[J];Food and Fermentation Industries;2003-02
7 GAO Jun\|ping1,LIANG Feng1,CHANG Jian\|hua1,GUO Li-an2,SU Tian-sheng3(1.Department of Chemistry, Northwest University, Xi′an 710069, China; 2.Central Laboratory,the Fourth Military Medical University, Xi′an 710032, China; 3.Beijing Institute of Chemis;Study on the Packings of Affinity Chromatography for the Separation of Urokinase[J];CHINESE JOURNAL OF CHROMATOGRAPHY;2000-02
8 Guo Li'an; Chen Wanlu; Zhu Baoquan; Chen Daijie(Central Laboratory,the Fourth Military Medical University, Xi 'an 710032);Synthesis of immunomagnetic microsphere and its separation for recombinant human IL-2[J];JOURNAL OF CELLULAR AND MOLECULAR IMMUNOLOGY;1999-01
9 He Binglin; Liu Yongning; Shi Zuoqing; Shi Rongfu; Zhang Quanxing; Feng Junqian and Fan Yunge (Iyistitute of Polymer Chemistry,Nankai University,Tianjin 300071);Application of an Adsorbent AB-8 in Extraction and Separation of Stevioside[J];CHINESE JOURNAL OF APPLIED CHEMISTRY;1994-01
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