Full-Text Search:
Home|About CNKI|User Service|中文
Add to Favorite Get Latest Update

Effects of Sulfated Polysaccharide from Masson Pine Pollen on Insulin Secretion and [Ca~(2+)]_i in MIN6 Cell

LIU Yue-ran1,2,FENG Wei,GENG Yue(1.Shandong Provincial Key Laboratory of Animal Resistance Biology,School of Life Sciences,Shandong Normal University,Jinan 250014,China;2.School of Medicine,Shandong University,Jinan 250012,China)  
The former research of our laboratory has found that the Pinus massoniana pollen polysaccharides precipitated by 60% alcohol(PPM60) and its sulfated derivative(SPPM60) could increase intracellular free calcium concentration in rat myocardial cells,splenocytes of mice and human chronic myelogenous leukemia cells.Intracellular free Ca2+ concentration can be one of the important factors affecting insulin secretion.However it is still unclear how about the PPM60 and SPPM60′s role in secretion of insulin of pancreatic β-cells,through changing the intracellular calcium concentration.This research aims at investigating the effects of masson pine pollen polysaccharide and its ester on insulin secretion and \i in MIN6 cell.Sulfated polysaccharide(SPPM60) was derivated from 60% ethanol precipitation of masson pine pollen polysaccharide(PPM60) modified by chlorosulfonic acid-pyridine method;enzyme-linked immunosorbent assay method was used to detect the insulin secretion in the MIN6 cells supernatant.MIN6 cells were treated with the calcium fluorescence probe Fura 2-AM,using a fluorescence spectrophotometer to detect \i changes affected by SPPM60.The concentration of insulin in MIN6 cell supernatant and \i in MIN6 cell was measured.Verapamil and Low Molecular Weight Heparin(LMWH) were used to testify whether insulin secretion and \i were affected.PPM60 had little impact on insulin secretion.However SPPM60 had significant role in promoting insulin secretion in MIN6 cell.SPPM60 increased \i significantly compared with the control group(P0.05).Verapamil and LMWH showed inhibition of insulin secretion caused by the SPPM60 in certain degree,but no significant difference.Similarly,they significantly inhibited the rise of \i caused by SPPM60(P0.05).Verapamil significantly inhibited the rise of insulin secretion(P0.05) and \i(P0.01) caused by glucose(3.6 mg/mL).SPPM60 could stimulate lonely the insulin secretion in MIN6 cell.It had similar mechanism to that of glucose-stimulated insulin secretion(GSIS) though there occured other signaling pathways.
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©CNKI All Rights Reserved