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《Pharmaceutical Care and Research》 2009-05
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Optimization of ultrasonic wave extraction technique of hypericin in Hypericum perforatum by orthogonal test

WU Di,DU Jia,CHEN Hui-yuan,ZHAI Zeng-wei,ZHOU Li-li(Biological Chemical Engineering Laboratory,School of Pharmaceutical Engineering,Shenyang Pharmaceutical University,Shenyang 110016,China)  
Objective: To establish an ultrasonic wave extraction technique of hypericin from Hypericum perforatum L..Methods:The influence of different factors such as category and concentration of solvent,fluid to material ratio,ultrasonic power,single radiation time and extraction time on the extraction efficiency was investigated.The technique of ultrasonic wave extraction was optimized by orthogonal test.Results:The optimal extraction conditions were as follows:extraction with 80% ethanol,material to fluid ratio of 1∶10(w/v),the ultrasonic power 400 W,single radiation time 3 s,extraction time 20 min per time.The extraction rate could reach(96.04±0.25)% and content of hypericin in the extract was 0.31%.Conclusion:This technique is simple and economical with high extraction rate,and the content of hypericin in extract fulfills the quality requirement.
【CateGory Index】: R284
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