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LI Zhi Xiao 1, HUANG Cheng Gang 2, CAI Yu Jun 3, CHEN Xiao Ming 1, WANG Fei 1, CHEN Yao Zu 1 (1.National Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; 2.Department of Chemistry, Zhejiang University, H  
AIM To study the structure and antioxidative activity of polysaccharide from Asparagus cochinchinensis . METHODS AND RESULTS the tube roots of Asparagus cochinchinensis was extracted with boiling water. From the extract, a polysaccharide, ACP 1 was separated using DEAE cellulose and purified by Sephadex G 150 column chromatography. Its structure was studied by composition analysis, IR, periodate oxidation analysis, Smith degradation, CrO 3 oxidation, methylation analysis, 1H and 13 CNMR analysis etc. ACP 1 was found to be composed of Gal and Glc, in the ratio of 1 00∶0 87, with the molecular weight of 10 4×10 4 and [α] 15 D=+64(c 0 54, H 2O). ACP 1 contained a backbone composed of 8β (1→4) linked D galactopyranosidic and 6α (1→6) linked D glucopyranosidic linkages, a glucosyl branch is attached to 3 O of a galactosyl residues of the main chain. The superoxide anion(O · 2) produced by NBT PMS NADH system was scavenged and the lipid peroxidation induced by Fe 2+ ascorbic acid in mouse liver microsome was inhibited, the hemolysis of rat RBC induced by H 2O 2 was antagonized. CONCLUSION ACP 1 is a galacto glucan and has distinctive effect of antioxidation.
【Fund】: 国家自然科学基金!重点资助项目 ( 3 973 0 480 )
【CateGory Index】: R914
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