Exendin-4 protected murine MIN6 pancreatic β-cells from oxidative stress-induced apoptosis via down-regulation of NF-κB-iNOS-NO pathway

LIU Li-bin~1,WANG Yan-ping~1,PAN Xiao-dong~1,JIANG Su-yuan~2,CHEN Zhou~3(1.Fujian Institute of Endocrinology,the Affiliated Union Hospital of Fujian Medical University,Fuzhou 350001,China;2.Shanghai Institute of Endocrinology,Shanghai Key Lab of Endocrine-Related Tumors,the Affiliated Ruijin Hospital of Shanghai Jiaotong University,Shanghai 200025,China;3.College of Pharmacy,Fujian Medical University,Fuzhou 350001,China)  

To explore the effect of glucagon-like peptide-1 receptor agonist-Exendin-4(Ex-4)on murine MIN6 pancreatic βcells apoptosis induced by oxidative stress,the morphological changes of cell damage were evaluated by epifluorescence microscopy after staining with AO-EB.The percentage of cell apoptosis was determined by flow cytometric assay after Annexin-V-FITC-PI staining.Nitric oxide level was measured by Griess reagent assay.Inducible nitric oxide synthase(iNOS) protein and NF-κBp65 fragment were detected by Western blotting.Ex-4 inhibited the increase of nitrite level and percentage of apoptosis induced by t-BHP in MIN6 cells.Furthermore,Ex-4 partly reduced the expression of iNOS protein and the ratio of NF-κBp65 protein in nucleus:cytosol induced by t-BHP.These results suggest that Ex-4 protects MIN6 pancreatic β-cells from oxidative stress-induced apoptosis via down-regulation of NF-κB-iNOS-nitric oxide pathway.

【Key Words】： Exendin-4 pancreatic β-cell apoptosis nuclear factor-κB inducible nitric oxide synthase nitric oxide tert-butyl hydroperoxide
【Fund】： 福建省高等学校新世纪优秀人才支持计划资助(NCETFJ-0703).
【CateGory Index】： R363
【DOI】： CNKI:SUN:YXXB.0.2008-07-003

Download(CAJ format) Download(PDF format)

CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.