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《Medical Recapitulate》 2015-24
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Effects of Saponins of Tribulus Terrestris on PPARγ and NF-κB Signaling Pathways Expression in Rat Brain Following Cerebral Ischemic Injury

ZHAI Feng-guo;LI Hou-zhong;ZHOU Fu-bo;LIN feng;GUAN Li-xin;Department of Pharmacology,Mudanjiang Medical University;  
Objective To study and explore the effect of tribulus terrestris on the expression of peroxisome proliferators γ( PPAR γ) and nuclear factor κB( NF-κB) inflammatory signaling pathways in rat brain tissue after focal cerebral ischemia,and further explore its potential mechanisms. Methods According to random number table method,40 SD rats were randomly equally divided into sham operation group,cerebral ischemia-reperfusion model group,low-dose saponins of tribulus terrestris group and high-dose saponins of tribulus terrestris group. Cerebral ischemia reperfusion model was established with suture emboli method in middle cerebral artery of rats. Neurological deficit scores was measured 24 hours after the cerebral reperfusion. Content of NF-κB,tumor necrosis factor-α( TNF-α) and interleukin-1β( IL-1β) in rat brain was detected by ELISA; expression levels of PPARγ protein in rat brain was determined by Western blot. Results Compared with the model group,nerve function injury of low dose tribulus terrestris saponin treatment group and high dose group obviously reduced[( 1. 8 ± 0. 7) scores,( 1. 3 ± 0. 5) scores vs( 2. 3 ± 0. 7) scores],the difference was statistically significant( P 0. 05). NF-κB,TNF-α and IL-1 β level of low dose saponins of tribulus group [( 16. 4 ± 1. 3) μg / mg,( 257 ± 110) pg / mg,( 148 ± 16) pg / mg]and high dose group[( 15. 0 ± 1. 2) μg/mg,( 665 ± 72) pg/mg,( 139 ± 14) pg/mg]were lower than those of the model group[( 18. 4 ± 1. 5) μg/mg,( 916 ± 128) pg/mg,( 169 ± 16) pg/mg]( P 0. 05). Conclusion Saponins of tribulus terrestris exerts the neuroprotective effect on cerebral ischemia-reperfusion injury in rats through inhibiting the inflammatory reaction,which may be associated with the increase of the PPARγ protein expression and inhibition of NF-κB inflammation signal pathway.
【Fund】: 黑龙江省自然科学基金(H201379);; 黑龙江省教育厅科学技术研究项目(11551527);; 黑龙江省卫生厅科研课题(2012-276)
【CateGory Index】: R285.5
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