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《Herald of Medicine》 2013-02
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Determination of Stability of Related Substances in Methylene Blue by TLC

LOU Yong-jun(Department of Chemical Drugs,Zhejiang Institute for Food and Drug Control,Hangzhou 310004,China)  
Objective To develop a thin-layer chromatography(TLC) method for determination of related substances in methylene blue and investigate the stability of it.Methods Five uL of test solution was applied to a suitable thin-layer chromatographic plate coated with a layer of chromatographic silica gel.The chromatogram was developed in a chromatographic chamber with a solvent system consisting of the upper layer separated from a well-shaken mixture of water,n-butanol and glacial acetic acid(100:80:20),and examined under ultraviolet light at 254 nm and natural light.Results Methylene blue underwent extensive degradation under thermal stress(60 ℃).It was found to degrade under thermal stress(40 ℃) and photolytic conditions.It possessed hygroscopicity and degraded under high humidity conditions(RH 92.5% and 75%).Conclusion The studies show that methylene blue is hygroscopic and unstable.
【CateGory Index】: R927
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 CHEN Si-qin reviewer WANG Ying-wei reviser(Department of Anesthesiology, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200092, China);Advance in clinical application of methylene blue[J];Journal of Shanghai Jiaotong University(Medical Science);2008-02
【Co-citations】
Chinese Journal Full-text Database 2 Hits
1 ZHENG Jia-peng,DING Zhen-qi,MENG Jia-rong,LIU Liang-tian.Department of Orthopaedic,175 Hospital of PLA,Zhangzhou,Fujian 363000,China;Experimental study of methylene blue intraarticular injection on knee joint of rabbit[J];Military Medical Journal of Southeast China;2013-03
2 LIAO Sheng-fang;CHEN Han-min;HUANG Guo-he;GUAN Ding-guo;WU Guo-xin;ZHANG Yi-wang;CHEN Shao-wei;Craniocerebral Injury Cure Center of Nanjing Command;Department of Neurosurgery, 180th Hospital of PLA;;Application research of methylene blue excretive test in the early stage of comprehensive nutrition support for severe hypertensive intracerebral hemorrhage[J];Chinese Journal of Clinicians(Electronic Edition);2013-21
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 YangRongjie,YinXingjia,ZhangXudong,etal.SecondHospital,ChinaMedicalUniversity,Shenyang110001.;Methylenebluestaininginfiberopticbronchoscopyinthediagnosisofbronchialtumors[J];Chiese Journal of Surgery;1996-03
2 Lai Bai—Tang, et al Department of Cytobiology, Buijing pulmonary Tumor Institute;ANTITUMOR EFFECT OF METHYLENE BLUE IN VITRO[J];Cancer Research on Prevention and Treatment;1990-01
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