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《Herald of Medicine》 2013-02
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Determination of Stability of Related Substances in Methylene Blue by TLC

LOU Yong-jun(Department of Chemical Drugs,Zhejiang Institute for Food and Drug Control,Hangzhou 310004,China)  
Objective To develop a thin-layer chromatography(TLC) method for determination of related substances in methylene blue and investigate the stability of it.Methods Five uL of test solution was applied to a suitable thin-layer chromatographic plate coated with a layer of chromatographic silica gel.The chromatogram was developed in a chromatographic chamber with a solvent system consisting of the upper layer separated from a well-shaken mixture of water,n-butanol and glacial acetic acid(100:80:20),and examined under ultraviolet light at 254 nm and natural light.Results Methylene blue underwent extensive degradation under thermal stress(60 ℃).It was found to degrade under thermal stress(40 ℃) and photolytic conditions.It possessed hygroscopicity and degraded under high humidity conditions(RH 92.5% and 75%).Conclusion The studies show that methylene blue is hygroscopic and unstable.
【CateGory Index】: R927
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