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《Chinese Journal of Applied & Environmental Biology》 2011-02
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Analyzing the Effect of Mixed Decomposition of Conifer and Broadleaf Litters on Soil Microbial Communities by Using PCR-DGGE

CHEN Falin1,ZHANG Kai1,ZHENG Hua1,LIN Xueqiang2,OUYANG Zhiyun1 & TU Naimei3(1State Key Laboratory of Urban and Regional Ecology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China)(2Hainan Research Academy of Environmental Sciences,Haikou 570206,China)(3College of Agronomy,Hunan Agricultural University,Changsha 410128,China)  
Clarifying the microbiological mechanism of litter decomposition is of great significance,especially in understanding how the soil microbial communities affect the decomposition of mixed litters with conifer and broadleaf trees.In this study,a simulation experiment was designed with flowerpots,and the litters of the typical species in the southern hilly red soil region,including Pinus massoniana(PM),Pinus elliottii(PE),Quercus fabri(QF) and Cyclobalanopsis glauca(CG),were investigated as study materials.The effects of the decomposition of the mixed litters on the genotypic diversity of soil microbial communities were studied by using PCR-DGGE method.The results indicated: 1) No significant change occurred in bacterial community structure and the lowest similarity was 72% if all treatments were graded as one class after mixing broadleaf litter with conifer litter.The differences were that the richness and diversity of 16S rDNA in PM+QF and PE+CG were significantly higher than that of single-conifer litter of PM and PE,respectively;2) Fungal community structure significantly changed and the similarity between the single-conifer litter treatment and the mixed litter treatment was only 28%.The richness and diversity of 18S rDNA in PM were significantly higher than those of PM+CG.The richness of 18S rDNA in PE was also significantly higher than that of PE+QF and PE+CG,and the diversity was significantly higher than that of PE+QF;3) The richness of 18S rDNA was positively related with initial carbon content of litter,but significantly negative with initial nitrogen content of litter.After mixing broadleaf litter with conifer litter,the proportion of nitrogen in litters increased,but the proportion of carbon declined significantly,which significantly affected the soil fungal community structure.
【Fund】: 国家自然科学基金项目(Nos.40871130 30600474)资助~~
【CateGory Index】: S714.336
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