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《Chinese Journal of Applied and Environmental Biology》 2019-05
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Biotransformation of phytosterols to 9α-hydroxyandrostenedione by Mycobacterium sp. LY-1 in an oil-water two-phase system

ZHOU Longfei;LI Hui;XU Yanan;ZHANG Xiaomei;SHI Jinsong;XU Zhenghong;School of Pharmaceutical Science, Jiangnan University;Key Laboratory of Industrial Biotechnology, Ministry of Education, College of Bioengineering, Jiangnan University;National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University;  
9α-Hydroxyandrost-4-ene-3,17-dione(9α-OH-AD),via the microbial conversion of phytosterols,is an important precursor in the manufacture of numbers of modern glucocorticoid and sex hormone drugs.The solubility of phytosterols was extremely low in water,and the bioavailability was not high,which seriously restricts the utilization efficiency of microorganisms on phytosterols.To increase the molar yield of 9α-OH-AD further,Mycobacterium sp.LY-1 was used as a starting strain by the previous mutagenesis,and the effects of the surfactants with different hydrophilic-lipophilic balance(HLB) values on the biotransformation efficacy of phytosterols to 9α-OH-AD were investigated.The most suitable surfactant was chosen and a highly efficient conversion system of oil-water(HLB=7.3) was established.The results showed that the surfactant Tween-80 with an HLB value of 15 increased the molar yield of 9 a-OH-AD.When 4.0 g/L Tween-80 was added,the molar yield of 9α-OH-AD reached 38.5%.Then,it was found that the addition of the solubilizing agent 100.0 g/L soybean oil promoted the transformation of phytosterol to 9α-OH-AD by Mycobacterium sp.LY-1.When 4.0 g/L Tween-80 and 100.0 g/L soybean oil were added,the molar yield of 9α-OH-AD reached 36.9%,and the quality concentration of 9 a-OH-AD was 8.17 g/L;this was 324.1% higher than the control treatment at a higher phytosterols concentration of 30 g/L.The results indicated that the oil-water conversion process system established in this study could increase the substrate concentration and improved the transformation ability of the Mycobacterium.This might provide a basis for industrial application.
【Fund】: 国家863计划重大项目(2011AA02A211);; 中国博士后科学基金面上项目(2018M640452);; 江苏省普通高校学术学位研究生科研创新计划项目(SJCX17_0502)资助~~
【CateGory Index】: TQ460.4
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