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《Evaluation and Analysis of Drug-Use in Hospitals of China》 2008-12
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Determination of Barbaloin in Aloe Ferox Mill by HPLC

LIU Yu-kui~(1),ZHAO Zuo-lian~2,GUO Ying-xia~2(1.Hebei Fengfeng Group Handan Hospital,Handan 056002,China;2.Hebei Handan Institute for Drug Control,Handan 056001,China)  
OBJECTIVE:To establish an HPLC method for the determination of barbaloin in Aloe ferox Mill.METHODS:The acetonitrile-water was taken as mobile phase(25∶75) at a detection wavelength of 355 nm.The samples were extracted,filtered and analyzed.RESULTS:The linear range was 0.400 8~4.008 μg·μL~(-1)(r=0.999 7) with a degree of precision of 0.7% and a recovery rate of 99.7%.The content of barbaloiin in Aloe ferox Mill was 20.1%.CONCLUSION:The determination method is simple and sensitive,and the determination results are reliable and accurate,thus this method is applicable for the content determination of barbaloiin in Aloe ferox Mill.
【CateGory Index】: R284
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