Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Applied Ecology》 2004-02
Add to Favorite Get Latest Update

Diversity of Frankia in nodules of Alnus nepalensis at Gaoligong mountains revealed by IGS PCR-RFLP analysis

DAI Yumei, CAO Jun, TANG Xiaomeng, ZHANG Chenggang(Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016,China)  
nodule samples were used to assess the diversity of Frankia strains symbiotically associated with Alnus nepalensis naturally occurring at the Gaoligong Mountains in Yunnan Province, China. DNA was extracted directly from nodules, and its two target DNA regions that encode nifD-nifK intergenic spacer (IGS) and 16S-23S rDNA IGS was studied by PCR-RFLP. The PCR fragments yielded by the nifD-nifK IGS were noticeably different in size, and when they were digested by HaeⅢ and AfaⅠ, 15 nif-type Frankia strains could be detected, the PCR-RFLP result of this region also could show that more than one genotype Frankia strains could form symbiosis with individual plants at the same time.The 16S-23S rDNA IGS had similar PCR fragments, but still identified 15 rrn-type strains after digested by HaeⅢ and AfaⅠ. 20 genotype strains could be found only when combined the PCR-RFLPs of two target regions.
【Fund】: 国家重点基础研究发展规划资助项目 ( 0 0 1CB10 89)
【CateGory Index】: S792
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 5 Hits
1 HAN Bin et al (Xishuangbanna Tropical Botanical Garden,Chinese Academy of Science,Kunming,Yunnan 650223);Study on the Nodule Nitrogen Fixation Ability of Alnus nepalensis Secondary Forest in Ailao Mountain[J];Journal of Anhui Agricultural Sciences;2009-17
2 XIONG Zhi1,LI Jie2,ZHANG Cheng-gang3(1.School of Forestry Resources,Southwest Forestry College,Kunming,Yunnan 650224,China;2.Key Laboratory for Microbial Resources of Education Ministry,Yunnan University,Kunming,Yunnan 650091,China;3.Institute of Applied Ecology,Chinese Academy of Sciences,Shenyang,Liaoling 110016,China);Correlations between host species of Alnus and endosymbiotic Frankia[J];Journal of Fujian Forestry Science and Technology;2008-01
3 Zhizhen Li1,2 (1The Academy of Fujian Forestry, Fuzhou 350012, China) (2Postdoctoral Research Station, College of Geographical Sciences of Fujian Normal University, Fuzhou 350007, China);Genetic Diversity of Frankia in Fujian[J];Acta Microbiologica Sinica;2008-11
4 HE Li-ping,MENG Guang-tao,LI Gui-xiang,CHAI Yong,FANG Xiang-jing,ZHANG Zheng-hai(Yunnan Academy of Forestry,Kunming,Yunnan 650204, China);Mineral Elements Distribution and Correlation in Alnus nepalensis and the Soil[J];Journal of Northwest Forestry University;2008-02
5 XIE Yi-qing~(1,2),LI Zhi-zhen~(1),HUANG Ru-zhu~(2),HUANG Yong~(1),XIAO Xiang-xi~(1)(1.Fujian Academy of Forestry,Fuzhou 350012,China;2.College of Bioengineering,Fujian Normal University,Fuzhou 350007,China);Effect of salt stress on growth and physiological and biochemical characteristics of Frankia[J];Journal of Plant Resources and Environment;2006-01
Chinese Journal Full-text Database 10 Hits
1 DONG Hairong,LI Cundong,LI Jincai(Agricultural University of Hebei,Baoding 071001, China);Effect of different NH~+_4/NO~-_3 ratios on young cotton growth and material accumulation[J];Journal of Agricultural University of Hebei;2003-01
2 ZHAO Yue,WEI Zi-min,LI Cheng,MA Feng-ming (Northeast Agricultural University,Life Science College,Harbin, Heilongjiang, 150030,PRC);The relationship between the key enzyme of nitrogen metabolism and sugarbeet yield and quantity[J];Journal of Northeast Agricultural University;2003-04
3 LI Yao,MA Chunmei,GONG Zhenping(College of Agriculture,Northeast Agricultural University,Harbin Heilongjiang 150030,PRC);Study on glutamine synthetase activity in spring soybean[J];Journal of Northeast Agricultural University;2006-06
4 Dai Yumei, Han Shijie, T ang Xiaomeng, Zhang Chenggang(Institute of Applied Ecology, Chinese Academy of S ciences, Shenyang 110016, P. R. China);Genetic Diversity of Frankia in Nodule of Alnus in Changbai Mountain Revealed by IGS PCR-RFLP[J];Journal of Northeast Forestry University;2003-06
5 Zhang Jie, Yang Chuanping(Northeast Forestry University, Harbin 150040, P. R. China); Zou Xuezhong(College of Forestry Professional Technology); Liu Changli(N ortheast Forestry University); Lu Jianzhou(Youjiang Forestry Bureau of Guangxi P rovince)Journal of Northeast Forestry University.;Relationships between Nitrate Reductase Activity, Chlorophyll, Soluble Protein Contents, and Growth Characters of Quercus mongolica[J];Journal of Northeast Forestry University;2005-04
6 Chen Yu 1,2 Zhu Baoge 1** Zhang Jing 1 Liang Zongsuo 2 (1. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Bejing,100101; 2.College of Life Sciences, Northwestern Sci-Tech University of Agriculture and Forestry,Yangling,712100);EFFECTS OF DIFFERENT NITROGENS ON ACTIVITIES OF NITRATE REDUCTASE,GLUTAMINE SYNTHETASE AND SEED PROTEIN VONTENTS IN SOYBEAN VULTIVARS[J];Soybean Science;2004-02
7 Zou Jijun 1 Dong Wei 2 Zhang Zhiyong 2 Chen Shouyi 2 Wang Jichai 3 Xu Jinxing 1 Yang Qingkai 1 Cao Yueping 1 (1 Soybean Research Institute, Northeast Agricultural University,Harbin, 150030 2 Institute of Genetics, Academia Sinica, Bei;STUDY OF AFFECTING FACTORS IN SOYBEAN RAPD[J];SOYBEAN SCIENCE;1998-03
8 ZHU Wan-ze~1,WANG Jin-xi~2,XUE Jian-hui~3 (1 Institute of Mountain Harzard and Environment,Chinese Academy of Science,Chengdu 610041,China;2 Academy of Sichuan Forestry,Chengdu 610081,China;3 Nanjing Forestry University,Nanjing 210037,China);Study on the photosynthetic characteristics of introduced Alnus formosana[J];Acta Botanica Boreali-occidentalia Sinica;2004-11
9 ZHU Wan-ze~1,WANG Jin-xi~2,XUE Jian-hui~3 (1 Institute of Mountain Harzard and Environment,Chinese Academy of Sciences,Chengdu 610041,China;2 Sichuan Forestry Academy,Chengdu 610081,China;3 Nanjing Forestry University,Nanjing 210037,China);Physiological Responses of Alnus formosana and Alnus cremastogyme Nursery Stocks of the Different Provenances to Water Stress[J];Acta Botanica Boreali-occidentalia Sinica;2005-10
10 CHEN Li-hong,YAN Wei,LIANG Jin-hui,LI Peng-fei(College of Agronomy,Inner Mongolia Agricultural University,Hohhot 010019);Genetic Diversity of Frankia Strains in Symbiosis with Hippophae rhamnoides in Inner Mongolia[J];Acta Botanica Boreali-Occidentalia Sinica;2006-12
【Secondary References】
Chinese Journal Full-text Database 3 Hits
1 CHEN Xin-hong et al(Department of Agronomy,College of Life Sciences and Engineering,Huaiyin Institute of Technology,Huaian,Jiangsu 223003);Effect of Salt Stress on Morphological and Physiological Characteristics in Wheat Seedlings.[J];Journal of Anhui Agricultural Sciences;2008-33
2 FENG Xian1,LI Da-wei2,CHEN Hong-wei1,3,YANG Bin1,SHI Fu-qiang1,CHEN Shao-an1,LI Jiang1,3,CHEN Wei1,3,MENG Meng1(1.Yunnan Academy of Forestry,Kunming Yunnan 650204,China;2.Southwest Forestry University,Kunming Yunnan 650224,China;3.Yunnan Provincial Key Laboratory for Forest Plant Cultivation and Utilization,Yunnan Laboratory for Conservation of Rare,Endangered & Endemic Forest Plants,State Forestry Administration,Kunming Yunnan 650204,China);Relevant Factors Screening for Superior Trees' Selection of Alnus nepalensis[J];Forest Inventory and Planning;2009-06
3 CAI Zu-guo1,ZHOU Hui-ping2,LI Hong-yun3,NIU Wen-kui3,ZHAO Yi-peng1(1.School of Horticulture and Landscape Architecture,Henan Institute of Science and Technology,Xinxiang,Henan 453003,China;2.Department of Life Science and Technology,Xinxiang University,Xinxiang,Henan 453003,China;3.The State Forestry Farm of Huanglianshu,Jiyuan,Henan 454650,China);Mineral Element Content of Endangered Paeonia suffruticosa var.spontanea[J];Journal of Northwest Forestry University;2010-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved