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《Acta Horticulturae Sinica》 1984-02
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PEROXIDASE ISOZYMIC ANALYSIS OF INTERVARIETAL HYBRIDS IN APPLE

Cheng Jiasheng Niu Jianzhe Di Shuyan ( Research Institute of Pomology, Chinese Academy of Agricultural Sciences )  
Materials used in this experiment were selfing or intervarictal hybrid seedlings of apple varieties 'Rails' ' Golden Delicious' and 'Delicious'. Isoperoxidases in cotyledons and leaves were separated by polyacrylamide gel electrophoresis. The results obtained indicated that characteristic ban-ds of the peroxidase isozyme in cotyledon or in leaf showed no significant differences, and that heredity conduct of the single isoperoxidase band conformed to Mendel's law of segregation. It was proved that peroxidase isozyme was controled by a single gene. According to the heredity cond-uct of the isoperoxidase PC band, it was proposed that 'Rails' and 'Gol-den Delicious' could be dominant heterozygote ( Pcpc ) , and 'Delicious' could be recessive homozygote ( pcpc ) , A biochemistic mutation in gene pc was found in the combination of 'Rails' × 'Delicious'. The Rate of mutation was about 1.3 per cent.
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