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《Acta Horticulturae Sinica》 1986-01
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PRELIMINARY STUDIES ON THE RELATIONSHIP OF SPECIES IN THE GENUS MALUS——ANALYSIS OF ISOPEROXIDASE

Cheng Jiasheng Liu Hanzhong Han Lixing Di Shuyan ( Research Institute of Pomology, Chinese Academy of Agricultural Sciences )  
Isoperoxidase of 482 samples from 13 species in the genus Malus was examined by polyacrylamide gel plate electrophoresis.The result obtained indicated that the isoperoxidase in Malus could be divided into 3 regions. In the first region of slow mobility, the zone of Rf value is from 0.10 to 0.30. In the second region of medium mobilization, Rf va-lue is 0.40-0.64. The third region has fast mobilization and the Rf va-lue equals 0.69-0.80. A total of 20 isoperoxidases distributed within the 3 distinct region were resolved. According to the frequency of each isozyme band in the second region, the basic zymogram of isoperoxidase in Malus was suggested. Further, a picture of the relationship between species or group of species was made by distance coefficient according to isoperoxidase zymogram.
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