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《Acta Horticulturae Sinica》 1989-01
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RAPID CLONAL PROPAGATION OF PHALAENOPSIS BY TISSUE CULTURE

Wang Huaiyiu ( The Floricultural Research Institute of Guangdong Academy of Agricultural Science, Guangzhou )  
Rapid clonal propagation of Phalaenopsis in vitro was studied.The vegetative buds were induced from lateral buds and the apices of Phalaenop-sis flower stalks in kyoto or Ms 3ppm BA medium. Shoot tips, stem sections and young leaves from in vitro plantlets formed protocorm-like-bodies (PLB) or adventitious buds in MS + 0.5-5ppm BA + lppm NAA or MS + 0.5-5ppm BA medium. When 5ppm BA + lppm NAA was added into the medium,65% of the stern sections formed adventitious buds,and 16.7% of the leaves formed PLB. In the MS + 1ppm BA medium, PLB sub-culture gave multiplication, and the structures formed young plants by further culture. The plantlets of the white flower clone Phal. ( phyllis key × band lea-der) × celebration have been transplanted on a large scale. The study has also shown that plantlets of Phalaenopsis developed by this method start flowering in 2-3 years after transplantation in Guangzhou area.
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