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《Acta Horticulturae Sinica》 2000-06
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Construction of a Genomic DNA Library of Citrus sinensis Osbeck

Chen Daming,Xu Changjie,and Zhang Shanglong (Horticultural Department,Zhejiang University,Hangzhou 310029)  
A genomic DNA library of citrus( Citrus sinensis Osbeck)was constructed in the study.Citrus genomic DNA was prepared with the CTAB method,and purified by CsCl spin.After the genomic DNA was partially digested with Sau 3A I,the first two nucleotides of this site were filled with Klenow.Then the fragments were ligated with LambdaGEM  12 Xho I Half Site Arms.The ligation mixture was packaged using the Packagene  extract and titrated on LB plates.A DNA library with 2.15×10 6 individual clones and a low background at 100pfu/ug DNA was obtained.The average length of inserts is about 17kb.The library was proved to be standard and representative.
【Fund】: 国家自然科学基金资助项目!(39770 5 2 6 ;39730 340 )
【CateGory Index】: Q943
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 Chen Daming; Zhang Shanglong ; Jin Yongfeng (Department of Horticulture, Zhejiang Agricultural University, Hangzhou 310029, China);A method for genomic DNA preparation of woody fruit crops[J];浙江农业大学学报;1997-06
【Co-citations】
Chinese Journal Full-text Database 6 Hits
1 LI Fangfang;YANG Shaozong;LIU Xinhong;LI haibo;WANG liling;LI Yonghua;Henan Agricultural University;Zhejiang Forestry Academy;;Genomic DNA extraction and establishment of the optimal SRAP-PCR reaction system for Liquidambar formosana Hance.[J];河南农业大学学报;2015-01
2 CHEN Hong;YANG Yi-ran;Guizhou Center for Fruit Engineering Technology;Research Institute for Fruit Resources of Karst Mountain Region of Guizhou University;College of Agriculture, Guizhou University;;Genetic diversity and relationship of plum resources in Guizhou analysed by ISSR markers[J];果树学报;2014-02
3 Liu Kangde; Li Jianguo; Peng Shiqin; Zheng Xueqin(Tropical Horticulture Institute, CATAS, Danzhou, Hainan, 571737; National Key Biotechnology Laboratory for Tropical Crops, CATAS, Haikou, Hainan, 571101);Genome DNA Extraction and RAPD Analysis of Avocado[J];热带作物学报;1999-04
4 Wang Sanhong,Chen Ligen and Chen Daming (Dept of Horticulture,Zhejiang Univ,Hangzhou 310029);Discrimination of nucellar seedlings of citrus with RAPD markers[J];南京农业大学学报;1999-03
5 Deng Jiusheng1 Chen Daming2 Peng Minzhang1 (1 Guangxi Vocational Technical College,Nanning5 30 2 2 7; 2 Zhejiang University,Hangzhou 31 0 0 2 9);Isolation and Appraisal of Chromosomal DNA from Longan and Litchi Fruit Trees[J];广西农业大学学报;1998-04
6 Jin Yongfeng;Zhang Yaozhoul;Chen Daming and Zhang Shanglong (Institute of Biochemistry, Department of Horticulture, Zhejiang Agricultural University, Hangzhon 310029);Identification of Peach Early-ripening Mutant 'Daguang 1' by RAPD Markers and Cloning of Specific Fragment[J];果树科学;1998-02
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