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《Acta Horticulturae Sinica》 2003-06
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Semi Vitro Culture of Pear Style and Identification of the Genotypes of Pear Self-incompatibility

Zhang Shading, Zhou Jiantao, Xu Yiliu, Chen Dixin, Xu Guohua, and Wu Guifa (College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China; Gaoyou Orchard, Gaoyou 225600, China)  
The results of semi vitro culturing pollinated styles excised from plants indicated that the amount of pollen tubes from style bottoms and the percentage of styles with pollen tubes through their bottoms in all tested styles differed with the cross combinations including the incompatible combination and compatible combination. For the former ones, the percentage ranged from 0 to 32.4%, most of which were under 16%, while for the latter ones, the percentage ranged from 51.6% to 86.7% that was close to the rate of field fruit setting after cross-pollination. Furthermore, when the pollen germination rate was above 20% , there was no significant difference between the flowers from the room water cultured flowering woods and field orchards as for the percentage of styles with pollen tubes through their bottoms in all styles. So we can extend the time span of collecting materials for our experiments. For the first time, the method of semi vitro culturing pollinated pear styles including cross compatible and cross incompatible was reported. We have also identified the genotypes of pear cultivars Chikusui, Kisui and Aikansui, namely S3S4,S4S5 and S4S5, respectively.
【Fund】: 国家自然科学基金项目(30170651);; 高校博士学科点专项科研基金项目(20010307012);; 江苏省自然科学基金项目(BK2001062);; 江苏省高技术研究计划项目(BG2002309)
【CateGory Index】: S661.2
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