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《Acta Horticulturae Sinica》 2006-01
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Metabolism of Dynamic Changes of the Reactive Oxygen in Tomato Pericarp and Seed Tissues during Fruit Ripening and Senescence

Ruan Ying,Liu Kailang,Shen Lin,Tian Huiqin,and Sheng Jiping~*(College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China)  
Tomato(Lycopersicom esculentum Mill.) pericarp and seed were used to investigate the changes of reactive oxygen and activities of antioxidant system enzymes during fruit ripening and senescence.(Superoxide) production rate(O~〖BHDFG1.5mm,WK1.5mmW〗_2),hydrogen peroxide(H_2O_2) content and the activities of superoxide dismutase(SOD),catalase(CAT),ascorbate peroxidase(APX) and glutathione reductase(GR) were determined.The degrees of the oxidative damage to the membrane lipids(MDA content) were also examined.The results showed that O~〖BHDFG1.5mm,WK1.5mmW〗_2 production rate and H_2O_2 content in seed were significantly higher than those in pericarp at all maturity stages,and the H_2O_2 content in the seed increased sharply after the pink stage.Higher activities of SOD,CAT and GR were observed in the seeds than in the pericarp.Higher APX activities were found from the BR stage to the RED stage in seed and that in pericarp.At the beginning of fruit ripening,an increase of CAT activity followed the accumulation of H_2O_2,but its activity declined quickly by the end of ripening,while that of APX and GR kept partial activities.These patterns of enzyme activity suggest that the AsA-GSH cycle might play an important role in scavenging H_2O_2.The maximum MDA content appeared in seeds earlier than in the pericarp,the maximum being 1.5 fold of that in the pericarp.High concentration of reactive oxygen in seed might be closely related with fruit ripening,and H_2O_2 might be an important factor in triggering the senescence.
【Fund】: 国家自然科学基金项目(30200191);; 国家‘十五’攻关项目(2001BA501A109B)
【CateGory Index】: S641.2
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