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《Acta Horticulturae Sinica》 2006-02
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Studies on the Methods of Establishing Cucumber Core Collection Based on RAPD Analysis

Zhang Guangping1,2,Li Xixiang1*,Xiang Changping2,Shen Di1,Wang Wenling3,and Song Jiangping1(1Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China;2College of Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070, China;3Haidian Science and Technology Extension Station of Beijing, Beijing 100871,China)  
The genetic diversity of 90 cucumber (Cucumis sativus L.) accessions from different countries or regions was detected by RAPD analysis. The methods of establishing the cucumber core collection based on RAPD data were studied. Firstly, six core collections were individually established at different sampling rate of 10%, 15%, 20%, 25%, 35% and 45% from the original collection. The various core collections were compared with the original collection in the number of polymorphic loci, number of lost loci and genetic diversity index. The results showed that 25% was more ideal for developing cucumber core collection in this study. Secondly, three sampling strategies(random sampling method, maximal deviation method, and maximal genetic distance method)were compared. The result demonstrated that the strategy of maximal genetic distance was better than others in establishing cucumber core collection with RAPD data. Further, it was verified that the core collection developed according to the sampling rate and strategy determined above could well represent the original collection, but at the same time, the reserve collection could not be neglected in germplasm conservation and utilization.
【Fund】: 北京市自然科学基金资助项目(6992023);; 农业部蔬菜遗传与生理重点开放实验室资助项目
【CateGory Index】: S642.2
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