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《Acta Horticulturae Sinica》 2008-10
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Pollen Germination Characteristic and Stigma Receptivity of Pugionium cornutum

HUANG Xiu-mei1, HAO Li-zhen1, HU Ning-bao1, ZHAO Qing-yan1, ZHANG Jin-wen1, WANG Liu-ying2, and TIAN Zhi-lai1(1College of Agronomy, Inner Mongolia Agricultural University, Hohhot 010019,China;2College of Ecology and Environment, Inner Mongolia Agricultural University, Hohhot 010019,China)  
Pollen germination characteristic of Pugionium cornutum (L.) Gaertner was evaluated by germination method on culture medium, its stigma receptivity was estimated by the hand cross-pollination and benzidine-H2O2 method. The results showed the optimal pollen germination of P.cornutum was on the culture medium added 20% sucrose, 0.001% boric acid and 0.5% agar. The effective temperature of pollen germination was from 15 ℃ to 38 ℃ and the optimal temperature was 25 ℃. The pollen germination rate was very higher from the biggest bud stage to 16 h after blooming, and the pollen viability was disappeared 48 h after blooming. The suitable condition of storing pollen was low temperature(4 ℃), and storing period was 7 d. The stigma of P.cornutum had receptivity from 48 h before blooming to 96 h after blooming,the strong receptivity in 24-72 h after blooming.
【Fund】: 国家自然科学基金项目(30460080 30260067);; 内蒙古科技攻关项目(20050305 20060202);; 内蒙古自然科学基金项目(2001108020501 200308020513);; 内蒙古人才基金项目
【CateGory Index】: S649
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