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《Acta Horticulturae Sinica》 2009-12
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The Effect of 5-azacytidine to the DNA Methylation and Morphogenesis Character of Chrysanthemum During in Vitro Growth

WANG Zi-cheng,NIE Li-juan,and HE Yan-xia(The Institute of Agriculture Biotechnology,Henan University,Kaifeng,Henan 475004,China)  
The effect of DNA methylation inhibitor(5-azacytidine,5-azaC) to chrysanthemum(Dendranthema×grandiflorum) has been studied by the method of in vitro treatment.The results indicated that 500 μmol·L-1 of 5-azaC caused death,above 100 μmol·L-1 of 5-azaC inhibited the materials’ development.The multiple buds differentiation of chrysanthemum was inhibited by every concentration of 5-azaC and the inhibiting effect of 5-azaC has time and dose additive effect.5-azaC treatment at low concentration induced the flowering early,this effect were stability and could be inherited through asexual multiplication.The results of MSAP(methylation sensitive amplified polymorphism technique) indicated that,the genomic of treated materials DNA methylation decreased distinctly.Part of the sites kept demethylation status in the successive transfer culture when 5-azaC removed.
【Fund】: 河南省教育厅自然科学基金项目(2009B210001)
【CateGory Index】: S682.11
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