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《Acta Horticulturae Sinica》 2010-05
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ISSR Analysis of Genetic Diversity of Lilium brownii var. viridulum Baker in Jiangxi Province

LI En-xiang1,HUANG Yu-qin1,JIANG Man-ying2,FANG Ming1,FU Chen-xi1,and YANG Bo-yun1, (1College of Life Science and Food Engineering,Nanchang University,Nanchang 330031,China;2College of Science,Nanchang University,Nanchang 330031,China)  
Lilium brownii var.viridulum Baker is an important medicinal plant species.Here we employed inter-simple sequence repeat (ISSR) markers to explore genetic diversity and patterns of genetic variation in 100 individuals from four cultivated populations in Jiangxi Province.Using 14 ISSR primers,213 discernible DNA fragments were scored with 165(77.5%)being polymorphic.High levels of genetic variation were revealed both at the population level(mean PPF = 31.69%,Ne = 1.198,I = 0.168,Hpop = 0.113)and the species level(PPF = 77.46%,Ne = 1.438,I = 0.392,Hpop = 0.260).Strong genetic differentiation among populations(AMOVA:ΦST = 0.632,P 0.001)was also detected,which may result from artifical selection and long-term cultivation.The UPGMA cluster analysis and principal components analysis (PCA) revealed that individuals from the same population formed a distinct group,and LS population was clearly separated from other populations.On the basis of these observations,some suggestions for efficient management of the genetic resources of this important medicinal herb are proposed.
【Fund】: 江西省教育厅项目(GJJ08059);; 江西省高校教改项目(JXJG-07-1-40)
【CateGory Index】: S644.1
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