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Development of Dominant Male Sterile Lines and Hybrid Seed Production in Cabbage ( Brassica oleracea var. capitata )

Fang Zhiyuan, Sun Peitian,Liu Yumei,Yang Limei,and Wang Xiaowu (Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081)  
A male sterile mutant 79-399-3 was obtained from the natural population of the original cabbage material 79-399. Its inheritance and utilization was investigated. The result indicated that the male sterility is controlled by a dominant gene CDMs399-3,which expressed in environment stable type and sensitive one under different gene backgrounds. Furthermore, temperature is the major factor to influence the sensitivity. Homologous male sterile plants were screened from the selfing progenies of the environment sensitive male sterile plants. After crossing the homologous male sterile plants with common inbred lines or fertile sister lines,populations with 100% male sterile plants were selected and their sterility degree was 100% or almost 100%. According to the charac teristics of CDMs399-3,a method was established to produce commercial hybrid seeds. The procedure included maintaining and propagating the homologous male sterile materials through tissue culture,and then producing desirable male sterile lines by crossing the homologous male sterile materials with characteristically similar inbred line or sib line. Hybrid seed production was conducted by crossing the male sterile line with inbred line which had good combining ability. The experimental seed production suggested that the male sterile line with CDMs399-3 gene had good seed setting.
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