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《Acta Nutrimenta Sinica》 1985-01
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METHODS OF FLUORIMETRIC DETERMINATION OF TRACE AMOUNT OF SELENIUM IN BIOLOGICAL MATERIALS,WATER AND SOIL 1.DETERMINATION OF SELENIUM IN HAIR,BLOOD AND URINE

Wang Guangya, Zhou Ruihua, Sun Shuzhuang, Yin Taian, Liu Shengjie, and Yang Guangqi(Institute of Health, Chinese Academy of Medical Sciences, Beijing)  
A modification of Watkinson's method was used for the flaorimetric determination of selenium in blood, hair, urine and animal tissues with 2,3-Di-aminonaphthalene. A mixture of sulphuric, perchloric acid and sodium molybdate was used for digestion. As little as 3 ng selenium in the sample could be estimated out. Coefficients of variation and recoveries for blood, hair, urine and animal tissues were 3.9, 5.5, 3.3 and 5.6%, and 97.0, 95.0, 97.8 and 99.8% respectively. No significant difference in selenium content estimated was found as graded amounts of samples were taken for analysis, indicating no foreign interference in the extracts. Both precision and accuracy of this method are satisfactory.
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