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《Acta Nutrimenta Sinica》 1986-02
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A RAPID METHOD FOR DETERMINATION OF TRYPTOPHAN IN FOODS AND FEEDSTUFFS WITH FLUORIMETRIC METHOD

Liu Yuequn, Hu Jian(Shanghai Food Research Institute, Shanghai)  
A rapid method for directly determining tryptophan contents in foods and feedstuffs was described. l-5mg dry sample could be completely hydro-lyzed in a teflon tube containing 5N NaOH with 0.5% soluble starch under 145℃ for 4 hours in vacuum (760mmHg). The hydrolysate was then adjusted to neutral with 6N HCL in icebath and diluted by five times with 4M urea solution (pH = 11), and was measured at excitation wavelength 280nm and fluorescence wavelength 368nm. The sensitivity of the method was 5ng /10ml, and the recovery rate of 97-107% were obtained in the range of 1-5 μg/10ml standard tryptophan. The value of a number of foods and feed-stuffs obtained were in agreement with the literatature values.
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【References】
Chinese Journal Full-text Database 10 Hits
1 WU Cai-lian,GUO Chang-jiang,YANG ji-jun,WEI jing-yu,XU Qi-shou(Institute of Hygiene and Environmental Medicine,Academy of Military Medical Science,Tianjin 300050,China);Separaton and Purification of L-Tryptophan from Fermentation Liquor[J];Amino Acids & Biotic Resources;2007-03
2 Jin Jingfang;Shen Mingquan(Shanghai Agricultural College 201101);Measurement of the Content of Tryptophan in Feather With Fluorescence Method[J];AMINO ACIDS & BIOTIC RESOURCES;1995-04
3 Zheng Bixing;Zhou Yun(Laboratory Centre,Guangxi Agric.Univ.,Nanning 530005);A STUDY ON THE METHODS OF TRYPTOPHAN DETERMINATION BY ANINO MID ANALYZER[J];;1994-02
4 Liao Shuihe She Xiaoge Fu Lixin Liu Jingzhi ( Jiangxi Analysing and Testing Institute, Nanchang, 330029, PRC );DETERMINATION OF AMINO ACIDS CONTENTS OF 63 KINDS OF FOODS OF JIANGXI PROVINCE[J];Jiangxi Science;1992-01
5 LI Jia sheng, CHEN Zhong ming (Inst. of Feed Sci.,Zhejiang University,Hangzhou 310029,China);Determination of Tryptophan in the Feedstuffs by HPLC[J];BULLETIN OF SCIENCE AND TECHNOLOGY;1999-03
6 Liu Shengyi(1. Department of Chemistry 2. Central Experimental 3. Microoganism Research Institute of Shandong University);CONCENTRATION OF AMINO ACIDS AND TRACE ELEMENTS Zn,Cu Mn AND Se OF ASPARAGUS OFFICINALISLINN[J];Acta Academiae Medicinae Weifang;1990-03
7 Zhang Guangyuan,Chen Yuzhen, Gu Chinfan (Institute of Hygiene and Environmentl Medicine, Tianjin 300050);Method of Determination of Tryptophan in Foods by Amino Acid Autoanalyzer[J];Acta Nutrimenta Sinica;1990-01
8 Liu Shengyi, Wang Xueyun,Li Lili,Wang Zhenhua(Department of Chemistryt Weifang Medical College; Microorganism Research Institute of Shandong University;Changwei Agricultural School);Concentrations of Amino Acids and Trace Elements Zn,Cu,Fe,Mn and Se of Asparagus Officinalis Linn[J];Acta Nutrimenta Sinica;1990-03
9 Zhao Jianxin, Liu Yuqun, Wang Guangming (Shanghai Food Research Institute, Shanghai 200233);STUDY OF RAPID HYDROLYSIS METHOD USING MICROWAVE AND NaOH SOLUTION TO DETERMINE TRYPTOPHAN IN FOOD[J];Acta Nutrimenta Sinica;1992-02
10 Teng Wei, Liu Qi(Central Laboratory, Shandong Academy of Agricultural Sciences, Jinan 250100)Li Jian, Lu Shaochun (Central Laboratory, Lai Yang Agricultural College, Lai Yang 265200);Amino Acid Contents of Taro[J];Acta Nutrimenta Sinica;1992-03
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 WANG Jian, CHEN Ning, TAN Qing-qiao, ZHANG Ke-xu (College of Food Science and Bioengineering, Tianjin University of Science and Technology, Tianjin 300222, China);Study on Breeding of L-tryptophan-producing Strain and Its Fermentation Conditions[J];Amino Acids & Biotic Resources;2003-03
2 Weng Lianjin 1,2 , Zou Jianhui 1, Gan Linhuo 1, Wang Shibin 1, Han Yuanyuan 1, Liu Caifu (1 Department of Chemical and Pharmaceutical Engineering, Huaqiao University, Quanzhuo 362021; 2 Quangang Nanyuan Chemical Co. Ltd., Quanzhou 362111);Study on Adsorption of L-Arginine on 732 Cation Exchange Resin and Extraction Process from Cystine Mother Liquor[J];Chemical Industry and Engineering Progress;2004-05
3 TANG Ai Guo, WANG Qing Ping (Department of Clinical Laboratory, Second Xiangya Hospital, Central South University, Changsha 410011,China);Determination of serum tryptophan by high performance liquid chromatography with fluorescence detection[J];Bulletin of Hunan Medical University;2002-06
4 SHAO Jian-juan~1,WU Hao~21. College of Pharmacy, Nanjing University of Traditional Chinese Medicine, Nanjing 210046, China;2. College of Life science and Pharmacy, Nanjing University of Technology, Nanjing 210009, China;Separation of L-Phenylalaning from Enzyme Production[J];Jiangsu Chemical Industry;2005-04
5 LIU Juxiang1 LIU Guodong1 YAN Husheng1 CHENG Xiaohui1 HE Binglin1 JIANG Genhua2 YANG Guoying2 1 The State Key Laboratory of Functional Polymer Materials for Adsorption and Separation, Institute of Polymer Chemistry, Nankai University, Tianjin 3000;DESALINATION OF MIXTURE OF AMINO ACIDS AND SALTS WITH ION EXCHANGE RESIN[J];Ion Exchange and Adsorption;2000-06
6 GAN Linhuo WENG Lianjin WANG Shibin Department of Chemical Engineering, Huaqiao University, Quanzhou Fujian 362011;RESEARCH ON THE PROPERTIES OF THE ADSORPTION OF L-ARGININE BY 732 CATION EXCHANGE RESIN[J];Ion Exchange and Adsorption;2002-06
7 Hu Yonghong;Ouyang Ringkai; Liu Xingrong (Department of Botechnology and Bioengineering, Nanjing Institute of Chemical Technology,Nanjina, China.210009);DETERMINATION OF TRYPTOPHAN IN ENZYMATIC SYNTHESIS[J];JOURNAL OF NANJING UNIVERSITY OF CHEMICAL TECHNOLOGY;1994-04
8 Liu Fang Xie Limin Shen Zhongyao (Department of Chemical Engineering, Tsinghua University, Beijing 100084);Study on Adsorption of L-tryptophan by Ion Exchange[J];Chinese Journal of Biotechnology;1996-04
9 LI Yu xia, WANG Shao heng (Department of Biology Science,Shihezi University,Shihezi 832003,China);A Study on the Determination Method of Trypotophan[J];Journal of Shihezi University(Natural Science);2001-01
10 Chen Ning; Sun Tao; Zhang Kexu(Department of Food Engineering, Tianjin Institute of Light Industry, 300222);The Breeding of L-Tryptophan Highly Producing Strains and Studies on Its Fermentation Conditions[J];FOOD AND FERMENTATION INDUSTRIES;1997-05
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 XIA Jun,CHEN Zhi-wen,SHI Ying(Departments of Biochemistry and Molecular Biology, Bengbu Medical College, Bengbu 233003, China);GREEN ASPARAGUS EXTRACTS INDUCE THE INHIBITION OF NUCLEIC ACID BIOSYNTHESIS IN TUMOUR CELLS[J];Carcinogenesis,Teratogenesis and Mutagenesis;2003-04
2 Zhao Jianxing;Liu Yuqun; Chen Wei(Shanghai Food Research Institute,200233);Amino Acid Analysis by Microwave High Pressure Vessel Hydrolysis[J];AMINO ACIDS & BIOTIC RESOURCES;1995-03
3 Liu Huiwen (The Research Institute of Forest Environmental Science, The Chinese Academy of Forestry, Beijing 10091);Determination of the Tryptophan in Cereal Crops with HPLC[J];AMINO ACIDS & BIOTIC RESOURCES;1997-04
4 Ding Changyu,Jiang Chengmei,Xin Jun,Zhao Hong,He Tianyuan,Duan Qiaoling (Department of Biochemistry,Bengbu Medical College,Anhui);ON ANTIAGING EFFECTS OF ASPARAGUS AND JUGLANs[J];ACTA ACADEMIAE MEDICINAE BENGBU;1995-04
5 Xia Jun;Chen Zhiweng;He Tianyuan;Zhao Xuehai;Jiang Bengkun(Deparlment of Biochemistry,Bengbu Medical College,Anhui 233003);Effect of green asparagus on human hepatoma Cell(BEL-7402)in vitro[J];ACTA ACADEMIAE MEDICINAE BENGBU;1996-02
6 Duan Qiaoling;Shi Ying; Xia Jun;Ding Changyu(Departnient of Biochemistry,Bengbu Medical College,Anhui 233003);Protective effect of asparagus on CCl_4-induced hepatotoxicity in mice[J];ACTA ACADEMIAE MEDICINAE BENGBU;1996-04
7 TIAN Hong-er 1, GAO Yong-sheng 2, ZENG Fu-li 2, LI Feng-min 2* , SHAN Lun 2 (1 The School of Public Health, Southeast University, Nanjing 210009, China;2 State Key Laboratory of Arid Agroecology, School of life Science, Lanzhou University, Lanzhou 730000, China);Effects of Eu~(3+) on Amino Acid and Protein Metabolisms in Lathyrus sativus[J];Acta Botanica Boreali-occidentalia Sinica;2005-06
8 Song Zhi-Feng1, Wang Li*1, Ji Feng1, Huang Huang1, Yu Zhi-Jing21(Soybean Research Center of Jilin Academy of Agricultural Sciences, Gongzhuling 136100)2(Graduate School of Jilin Agricultural University, Changchun 130118);Rapid Determination of Amino Acids in Feedstuffs by High Performance Liquid Chromatography Using Internal Standard Method with Online Pre-column Derivatization[J];Chinese Journal of Analytical Chemistry;2007-01
9 Hu Zhengzhi (Scientific Research Institute of Food and Fermentation Industry. Ministry of Light Industry. Beijing 100027),;The Analysis of Foods[J];Analytical Laboratory;1992-05
10 Jiang Shiqiang,Dong Muxin and Xie Faming (Chinese Academy of Agriculturai Sciences, Beijing 100081),;Analysis of Agricultural sample[J];Analytical Laboratory;1992-06
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