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《Acta Nutrimenta Sinica》 2002-04
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EFFECT OF PHYTOESTROGENS ON THE PROLIERATION OF MCF-7 IN VITRO

YU Zeng-li, ZHANG Li-shi, WU De-sheng (Huaxi School of Public Health, Sichuan University,Chengdu 610041,China)  
Objective: The purpose of the study was to explore the effects of phytoestrogens on the proliferation of breast cancer MCF-7 cells. Methods: Human estradiol-dependent breast cancer MCF-7 cells were grown in DMEM medium containing 10% bovine serum. Five days before addition of the test compounds, the cells were washed with phosphate-buffered saline, and then cultured in phenol red-free DMEM medium containing 5% dextran charcoal-stripped FBS. The test compounds (genistein and zea-ralenone) were added in fresh medium and the control cell received only the vehicle (ethanol). The proliferation of MCF-7 was analyzed by MTT assay, 3H-TdR incoporation assay and flow cytometry. Results: Compared with the EtOH control group, the proliferation of the cells treated with genistein(8 μmol/L)was markedly restrained. Zearalenone, like estradiol, could markedly increase the proliferation of MCF-7 cells. The effects were time-dependent and dose-dependent. Conclusion: The tested phytoestrogens are biologically active, and they can differently affect the proliferation of human breast cancer cells in vitro. These data suggest that genistein may have preventive and therapeutic applications against breast tumors.
【CateGory Index】: R737.9
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