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《Acta Nutrimenta Sinica》 2006-02
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YU Zeng-li, ZHANG Li-shi 1, WU De-sheng 1 ( School of Public Health, Zhengzhou University, Zhengzhou 450052;1Huaxi School of Public Health, Sichuan Universitya, Chengdu 610041, China)  
Objective: To characterize the effects of genistein (GS) on tumor-associated gene expression in MCF-7 cells and to explore the mechanisms. Method: MCF-7 cells were treated with GS (75×10-6 mol/L) or with vehicle (0.1% ethanol, EtOH) for 72 h. The cells were collected and total RNA was extracted, marked by two different fluorescence dyes (Cy3 and Cy5) using reverse transcriptional reaction, respectively. The derived cRNA was hybridized to human tumor-associated microarrays. Data were processed using the Scan Array 3000 and ontological analysis was performed using the Ima Gene 3.0 software. Results: At 72 h, 18 transcripts were downregulated compared to EtOH vehicle control by greater than 2- fold and 4 were upregulated by less than 0.5-fold. The predominant ontological groupings were oncogenes, cell proliferation, apoptosis, and estrogen-regulation genes. Conclusion: GS treatment is associated with significant changes in gene expression in several functional categories in MCF-7 cells, and this might account for the role of genistein in prevention of breast cancer.
【Fund】: 国家自然科学基金(No.30030120)
【CateGory Index】: R737.9
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Chinese Journal Full-text Database 2 Hits
1 Yu Zengli, Zhang Lishi, Wu Desheng School of Public Health, University Zhengzhou, Zhengzhou 450052,China;Effects of three environmental estrogens on expression of proliferation and apoptosis-associated genes in PEO4 cells[J];Journal of Hygiene Research;2004-04
2 YU Zeng-li, ZHANG Li-shi, WU De-sheng (Huaxi School of Public Health, Sichuan University,Chengdu 610041,China);EFFECT OF PHYTOESTROGENS ON THE PROLIERATION OF MCF-7 IN VITRO[J];Acta Nutrimenta Sinica;2002-04
Chinese Journal Full-text Database 10 Hits
1 LI Zheng.Department of Nutrition,the First Central Hospital,Tianjin 300192,China;Research Advance on Biological Effects of Genistein[J];Journal of Environment and Health;2008-03
2 NIU Jian-zhao1,ZHAO Pi-wen1,WANG Ji-feng1,YU Jie1,WANG Da-wei1,SHEN Li-xia1,2(1 School of Fundamental Medicine,Beijing University of Chinese Medicine,Beijing 100029;2 North University of Hebei);Phytoestrogenic activity of five kinds of Chinese herbal medicines including Fructus Psoraleae[J];Journal of Beijing University of Traditional Chinese Medicine;2008-10
3 ZHANG Guo-fu,LI Zhong*,MO Bao-qing,WEI Wei(Department of Nutrition and Food Science, School of Public Health of NJMU, Nanjing 210029, China);The effects of genistein on the proliferation and cell cycle in human breast cancer cell MCF-7[J];Acta Academiae Medicinae Nanjing;2005-05
4 SUN Jie,WANG Shao,GUO Bin,WEI Ya-hui (Key Laboratory of Resource Biology and Biotechnology in Western China,Ministry of Education,College of Life Science, Northwest University,Xi'an 710069,China);Suppressive Effect of Rhizoma sparganii Flavonoids on HeLa Cells (I):Induce M Stage Arrest and Cell Apoptosis[J];Food Science;2011-01
5 ZHENG Ji-cui,MA Yang-yang,LIU Jiang-bin,XIAO Xian-min(Department of Surgery,Children's Hospital,Fudan University,Shanghai 201102,China);Effect of bisphenol A and zearalenone on the expression of cell cycle protein in SK-N-SH human neuroblastoma cells[J];Fudan University Journal of Medical Sciences;2011-01
6 YU Zeng-li~1, ZHANG Li-shi~2, WU De-sheng~2(1.School of Public Health, Zhengzhou University,Zhengzhou Henan 400052,China);The effects of zearalenone on tumor-associated gene expression in MCF-7 cells[J];Journal of Health Toxicology;2005-03
7 YU Zeng-li,HAN Ping,LI Wen-jie (Shool of Public Health, University of Zhengzhou 450052 China);STUDY ON THE PROMOTIVE EFFECT OF GENISTEIN ON BONE FORMATION[J];Acta Nutrimenta Sinica;2005-01
8 YU Zeng-li,ZHANG Li-shi1,WU De-sheng1 ( School of Public Health, University Zhengzhou 450052; 1 School of Public Health, University Sichuan ,610041 China );THE MECHANISMS OF PROLIFERATION AND APOPTOSIS IN MCF-7 CELLS BY DIETARY ESTROGENS[J];Acta Nutrimenta Sinica;2005-02
9 ZHANG Xing-guo,ZHAO Qing-wei (First Affiliated Hospital,College of Medicine,Zhejiang University, Hangzhou 310003,China);Estrogenicity of adzuki bean Phaseolus angularis wight.and its effect on proesterone receptor level in human breast cancer MCF-7 cells[J];China Journal of Chinese Materia Medica;2006-15
10 LI Bin-bin, YU Shi-feng, PANG Shu-zhen. Department of Oral Pathology, Peking University School of Stomatology, Beijing 100081, China;Effect of genistein on the proliferation, differentiation and apoptosis of the osteoblasts[J];Chinese Journal of Stomatology;2005-03
China Proceedings of conference Full-text Database 2 Hits
1 ;植物雌激素作用机制的研究现状及有关进展[A];[C];2008
2 ;不同产地、种属十种卷柏雌激素活性筛选的实验研究[A];[C];2008
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 YU Zeng-li,ZHANG Li-shi,LIU Fu-yun,et al.Huaxi School of Public Health,Sichuan University,Chengdu,Sichuan610041,China;Effects of Environmental Estrogens on Apoptosis of Ovarian Cancerous PEO4 Cells[J];Journal of Environment and Health;2003-03
2 YU Zeng-Li,ZHANG Li-shi,WU De-sheng.Huaxi School of Public Health,Sichuan University(Chengdu 610041,China);Effect of Environmental Disruptors on Prolieration of Ovarian PEO_4[J];China Public Health;2002-12
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