EFFECTS OF MOGROSIDE EXTRACT ON CELLULAR IMMUNE FUNCTIONS IN ALLOXAN-INDUCED DIABETIC RATS
CHEN Wei-jun,SONG Fang-fang1, LIU Lie-gang1,QI Xiang-yang,XIE Bi-jun,SONG Yun-fei2 (Department of Food Science and Technology , Huazhong Agricultural University, Wuhan 430070; 1Department of Nutrition and Food Hygiene , Tongji Medical College , Huazhong Science and Technology University, Wuhan 430030,,2Guilin Layn Natural Ingredients Corp,Guilin 541300 )
Objective:To study the effect of mogroside extract on splenic lymphocytes subsets and the cytokines expression levels of type 1 diabetes mellitus (TIDM) mice. Method:Balb/c mice were rendered diabetic by a single intra-peritoneal injection of alloxan, at dose of 200 mg/kg bw. After confirmation of TIDM, control and diabetic mice were further subdivided into the following six groups: control (C), control + low dose MG (150 mg/kg bw, C-LMG), control + high dose MG (300 mg/kg bw, C-HMG), diabetic (D), diabetic+ low dose MG (150 mg/kg bw, D-LMG), diabetic +high dose MG (300 mg/kg bw, D-HMG). The MG or saline was administered by gavage daily over 30 d. At the end of the experiment, determinations of serum glucose concentration, splenic lymphocyte subpopulation and cytokines production (IFN- , TNF- and IL-4) and histopathological examinations of pancreatic islets were carried out. Results:TIDM mice presented hyperglycemic manifestation and significant injury in islets. Besides, alloxan induced a markedincrease in the CD8+ expression (thus a dramatic decrease in CD4/CD8) and production of IFN- and TNF- in splenocytes. The MG, especially at the low dose effectively attenuated the early clinical symptoms, biochemical abnormalities, pathological damages in pancreatic islets and regulated the immune dysfunction in alloxan-induced TIDM mice, possibly by influencing (up-regulating) the CD4+ T lymphocyte subsets and CD4/CD8 ratio, and remodeling the intracellular cytokine profiles (reducing the expression of pro-inflammatory Th1 cytokines: IFN- , TNF- towards a beneficial Th2 pattern). MG therapy had no obvious effect on normal mice, except that at the low dose MG could up-regulat the IL-4 expression . Conclusion:MG could exhibit anti-diabetic effects, due to its immunity-regulating capacity on TIDM, and may be used as a new natural drug for prevention and treatment of TIDM.