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《Acta Nutrimenta Sinica》 2010-05
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STUDY ON THE EFFECT OF FLUORIDE ON CULTURED ENDOTHELIAL CELLS AND THE ROLE OF SELENIUM INTERVENTION

BIAN Jian-chao,LIN Xin-ying1,YANG Xiao-xia,HOU Xiao-dong1,FAN Ting,ZHU Qiu-li1(Shandong Institute for Prevention and Treatment of Endemic Disease,Jinan 250014;1Department of Nutrition and Food Hygine,School of Public Health,Shandong Universgy,Jinan 250012,China)  
Objective To study the influence of fluoride in different concentrations on cultured vascular endothelial cells and the effect of selenium intervention.Method Human umbilical vein endothelial cells(HUVEC) were cultivated at 37 ℃,5% CO2,saturated humidity conditions.The cell culture medium was added by sodium fluoride,at fluoride concentrations 0,100,400,1000,2000 μmol/L,while selenium was added at 100 nmol/L.Cell culture medium and cells were collected after 48h continuous incubation.Wright-Giemsa staining was applied to observe cell morphology,acridine orange fluorescence staining for cell apoptosis,and MTT colorimetry assay for cell activity.The anti-oxidation parameters as superoxide dismutase(SOD) of cytolymph,glutathione peroxidase(GSH-Px) activity,malondialdehyde(MDA) content were determined.Inducible nitric oxide synthase(iNOS) and endothelial nitric oxide synthase(eNOS) activities,cell iNOS-mRNA and eNOS-mRNA expressions and intercellular adhesion molecule(ICAM-1) and vascular adhesion molecule(VCAM-1) contents were detected.Results Adding 100 nmol/L Se to fluoride concentration of 100-700 μmol/L can reduce the inhibition of fluoride on cell growth significantly and restore the change of cell morphology.SOD and GSH-Px activities were increased(P0.05),MDA content decreased(P0.05),iNOS activity and iNOS-mRNA expression reduced(P0.05),eNOS activity and eNOS-mRNA expression increased(P0.05);and CAM-1 and VCAM-1 content reduced(P0.05,P0.01).Conclusion Fluoride can induce vascular endothelial cell morphological changes and functional abnormalities.Selenium can improve the anti-oxidation function,adjust NO metabolism,inhibit abnormal expression of cytokines,as antagonist of vascular endothelial injury caused by high-fluoride.
【Fund】: 山东省人民政府、山东省国土资源厅资助(No.1212010310306)
【CateGory Index】: R599
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