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《Acta Nutrimenta Sinica》 2011-01
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EFFECTS OF WATER EXTRACT OF PROPOLIS ON LUNG INJURY AND NF-κB/ICAM-1 PATHWAY IN LUNG FOLLOWING INTESTINAL ISCHEMIA REPERFUSION IN RATS

YAO Shu-tong,SANG Hui,SHANG Zhan-ping,TIAN Hua,FANG Yong-qi,WANG Jia-fu(Department of Pathophysiology,1Institute of Atherosclerosis,Taishan Medical College,Taian 271000,China)  
Objective To investigate the protective effect of water extract of propolis(WEP) on lung injury induced by intestinal ischemia-reperfusion(I/R) in rats and its mechanism.Method Thirty-two male Wistar rats were randomly divided into sham,I/R and WEP(100,200 mg/kg) pretreated groups.The model of intestinal I/R injury was made by clamping super mesenteric artery for 45 min followed by 120 min of reperfusion in rats.At the end of the experiment,the changes of arterial blood gas level,lung index and myeloperoxidase(MPO) activity in lung tissue were measured,and the morphological changes of lung tissue were observed through light microscopy.The content of nuclear factor-κB p65(NF-κB p65) and intercellular adhesion molecule-1(ICAM-1) in lung tissue were detected by immunohistochemical staining and Western blotting.Results The respiratory function was improved in WEP groups,charactered by the increase of PaO2 and decrease of PaCO2(P0.05 vs I/R group).The lung index was decreased(P0.05) and the pathologic lesion of lung tissue was alleviated significantly by WEP,compared with those in I/R group.In addition,WEP inhibited the increase of MPO activity(P0.05) and the over-expression of NF-κB p65 and ICAM-1 in lung tissue induced by intestinal I/R in dose-dependent manner(P0.05 or P0.01).Conclusion WEP attenuates lung injury induced by intestinal I/R in rats,which is due to its suppression for the activation of NF-κB and subsequent neutrophils accumulation mediated by ICAM-1.
【Fund】: 泰山医学院重点资助项目(No.2007ZR001);泰山医学院青年基金项目(No.2009ZRQN033)
【CateGory Index】: R285
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