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《Acta Nutrimenta Sinica》 2012-06
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INFLUENCE OF SAPONIN OF PEARSONOTHURIA GRAEFFEI ON HEMATOPOIETIC MODULATORS IN MICE

LIU Dong,WANG Jing-feng,LI Wei-hang,LI Bing,WANG Yu-ming,XUE Chang-hu(College of Food Science and Engineering,Ocean University of China,Qingdao 266003,China)  
Objective To evaluate the influence of saponin of Pearsonothuria graeffei(PGS) on hematopoietic modulators in myelosuppression mice.Method Male ICR mice were randomly divided into four groups: normal control group,model control group,low and high dose PGS groups.PGS was orally administered with 10 and 30 mg/kg.bw every day for 16 d.The mice in normal and model control groups were administered with 0.9% NaCl solution as placebo.On the 7th day,all mice except those in the normal group were injected intraperitoneally with 100 mg/kg cyclophosphamide once a day for 3 d to induce myelosuppressed animal model.The lung conditioned medium(LCM),spleen cell conditioned medium(SCM) and peritoneal cavity macrophage conditioned medium(PMΦCM) treated with Pearsonothuria graeffei were prepared to observe the effects on proliferation of bone marrow cells and formation of hematopoietic progenitor cells colonys including granulocyte-monocyte colony forming units(CFU-GM),colony-forming units-erythroid(CFU-E) and colony forming unit-megakaryocyte(CFU-Meg) by MTT.The mRNA expressions of granulocyre-macrophage colony-stimulating factor(GM-CSF),erythropoietin receptor(EPOR) and thrombopoietin(TPO) were determined by semi-quantity RT-PCR.Results PGS could significantly promote the proliferation of bone marrow cells,the colony formation of CFU-GM,CFU-E,CFU-Meg in normal mice,but each colony showed various features.PGS could increase mRNA expression abundance of GM-CSF,EPOR and TPO in bone marrow cells.Conclusion Saponin can promote effectively the hematopoietic function by up-regulating level of hemopoietic growth factors in myelosuppressed mice.[ACTA NUTRIMENTA SINICA,2012,34(6):599604]
【Fund】: 海洋公益性行业科研专项(No.201105029);; 国际科技合作项目(No.2010BFA31330);; 国家自然基金(No.31000795);; “泰山学者”建设工程专项经费
【CateGory Index】: R285.5
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