Expression of Marek's Disease Virus pp 38 Gene in Insect Cells with Use of Baculovirus as a Vector
Cui Zhi-zhong;L. F. Lee Jiangsu Agricultural College, Yangzhou, 225001 Avian Disease and Oncology, Laboratory, USDA, 3606 East Mt. Hope Road, East Lansing, Michigan 48823, USA
The complete encoding sequence with the initiation and stop codons ofMarek's disease virus(MDV) 38 kd phosphorylated protein (pp38) gene wasintegrated into the baculovirus AcNPV transfer vector pVL1392. The insectcell line Sf9 cells were cotransfected with the recombinant transfer vectorpVLpp38 I and the wild type AcNPV DNA. A recombinant baculovirus cloneBP38 I, which was expressing the MDV pp38 gene, was screened with theanti-MDV monoclonal antibody (MAb) H19 in fluorescence antibody test.Immunoblot indicates that the MAb H19 could recognize a MDV-specific pro-tein band of 35--36 kd from the recombinant virus BP38 I -infected Sf9 celllysates.