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《The Chinese Journal of Dermatovenereology》 2011-06
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Prokaryotic Expression and Immunogenicity of the CT703 Protein from Chlamydia Trachomatis

DU Kun1,YU Ping2,WEI Yun-mei1,ZHENG Qun1(1.Clinical Laboratory,the First People's Hospital of Jingzhou City,Jingzhou 434000,China;2.Xiangya School of Medicine,Central South University,Changsha 410078,China)  
Objective To construct the prokaryotic expression plasmid pGEX/CT703,express CT703 protein and study its immunogenicity in mice.Methods The entire DNA fragment of CT703 gene was obtained from the Chlamydia Trachomatis(CT) serovar L2 genome by RT-PCR,and was subcloned into the pGEX plasmid.The recombinant plasmid was transformed into competent E.coli BL21 to express GST-CT703 fusion proteins after identification by restriction enzyme digestion and sequence analysis.The GST-CT703 fusion proteins were used to immunize BALB/c mice.ELISA and Western blot were carried out to identify the immunogenicity and immunoreactivity.Results The recombinant expression plasmid pGEX/CT703 had been constructed successfully,which was demonstrated by restriction enzyme digestion and sequence analysis.The expressed fusion protein induced by IPTG had a relative molecular mass of 81KD.Western blot assay demonstrated the antiserum bound specifically CT703 protein,and ELISA result showed the specific antibody titer of CT703 reached 1:320 00.Conclusion CT703 protein can be expressed successfully in E.coli BL21 and the specific anti-CT703 antibody is induced in BALB/c mice.
【CateGory Index】: R392
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 LIU Yuan-jun,YAO Wei-feng,HOU Shu-ping,QI Man-li,WANG Hui-ping,LIU Quan-zhong(Department of Dermatology,Tianjin Medical University General Hospital,Tianjin 300052,China);Cloning,Prokaryotic Expression and Identification of Chlamydial GPIC Capsid Vp2 Protein[J];The Chinese Journal of Dermatovenereology;2010-06
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 LIU Yuan-jun,QI Man-li,SHAO Li-li,SHENG Cai-hong,YAO Wei-feng,HOU Shu-ping,LIU Quan-zhong(Department of Dermatology,General Hospital of Tianjin Medical University,Tianjin 30052,China);Comparison of the Differences among the Standard Strains of Chlamydia Trachomatis[J];The Chinese Journal of Dermatovenereology;2009-07
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