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《Chinese Journal of Pathophysiology》 2001-05
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Human mesenchymal stem cells differentiate into neuron-like cells

XIANG Peng 1,XIA Wen-jie 1,ZHANG Li-rong 2,CHEN Zhen-guang 3, ZHANG Xiu-ming 1,LI Yan 1, LI Shu-nong 1 ( 1Department of Pathophysiology, Sun Yat-sen University of Medical Sciences, Guangzhou 510080, China; ? 2?Department of Pathophysiology,  
AIM: To investigate the differentiation from human mesenchymal stem cells(hMSC) into neuron-like cells.METHODS: hMSC were separated from rib marrow with Ficoll-Paque reagent and expanded in culture medium. hMSC were induced to differentiate with DMEM/monothioglycerol or DMEM/β-mercaptoethanol, respectively. Neuron-specific enolase(NSE), neurofilament(NF), and glial fibrillary acidic protein(GFAP) were detected by immunohistochemistry. RESULTS: hMSC were expanded as undifferentiated cells in culture for more than 5 passages. When treated with monothioglycerol or β-mercaptoethanol for 5 hours, hMSC exhibited neuronal phenotype. The expression of NSE and NF in the neuron-like cells was positive, but the glial astrocyte marker GFAP didn't express. CONCLUSION: hMSC can be induced to differentiate into neurous.
【Key Words】： Stem cells Neurons Nervous system diseases
【Fund】： 国家重点基础研究发展规划项目! (973)资助(No .G19990 5 430 0 )
【CateGory Index】： R329.2
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