Study on the potential to differentiate into myocytes of the CD34~+ cells

CHEN Yun-xian 1, ZHONG Xue-yun 2, OU Rui-ming 1, CHEN Hui-zhen 1, LUO Wei-qiong 3, ZHONG Li-ye 1, XING Da 4, HAN Zhong-chao 5 (? 1?Department of Internal Medicine, The First Affiliated Hospital, Zhongshan University, Guangzhou 510080, China;  

AIM:To investigate the potential of differentiatng into myocytes of the granulocyte colony-stimulating factor(G-CSF)-mobilized CD34 + cells. METHODS: Three hours after intraperitoneal injecction of isoprenaline(ISO) to develop acute ischemic model, rats' bone marrow hematopoietic stem cells were mobilized to the site of myocardial infarction by G-CSF. The techniques of immunohistochemisty and HE stain were used to detect the infiltration of CD34 + cells and the regeneration of myocytes in the infarct zones. RESULTS: 24 hours after administration of ISO , a large amount of infiltrative monocytes and regenerative myocytes which were CD34 positive expression could be found in the infarct zones of the G-CSF treatment group, while majority of the infiltrative inflammatory cells in control group were neutrophils and there was no infiltrative cells and myocytes which were CD34 positive expressio, 2 weeks after administration of ISO, there were a plenty of scar in control group, but not in the G-CSF treatment group. CONCLUSION: G-CSF-mobilized CD34 + cells possess the potential to differentiate into myocytes and it may be used in treating acute myocardial infarction.

【Key Words】： CD34 + cells Myocardial infarction Cell differentiation Granulocyte colony-stimulating factor Rats
【Fund】： 国家攀登专项计划项目 ( 95 -专 - 10 ) ;; 广东省科技厅团队项目 ( 0 190 12 );; 广东省“五个一”科教兴医工程重点项目 (粤卫 1996- 186号 )
【CateGory Index】： R329.2

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