Full-Text Search：
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Pathophysiology》 2003-07
Add to Favorite Get Latest Update

Human mesenchymal stem cells differentiate into neuron-like cells with Tanshinone II A

XIA Wen-jie 1, XIANG Peng 1, ZHANG Li-rong 2,CHEN Zhen-guang 3, YU Wei-hua 1, ZHANG Xiu-ming 1, LI Yan 1, LI Shu-nong 1 (? 1?Department of Pathophysiology,Sun Yat-sen Medical College, Sun Yat-sen University, Guangzhou 510080, China;? 2?Department of Pathophysiology, Guangdong Pharmcology College, Guangzhou 510224, China;? 3?Department of Cardiothoracic Surgery, First Affiliated Hospital, Sun Yat-sen University ,Guangzhou 510080, China)  
AIM:To investigate the differentiation from human mesenchymal stem cells(hMSC) into neuron-like cells with Tanshinone II A. METHODS: hMSC were separated from rib marrow with Ficoll-Paque reagent and expanded in culture medium. To detect the surface antigens, the labeled cells were analysed on a FACScan flow cytometer to determine the effect of the capacity of proliferation and differentiation of the mesenchymal stem cells with FGF-2. hMSC were induced to differentiate into neurons with DMEM Tanshinone II A. Neuron-specific enolase(NSE), neurofilament(NF), Nestin, glial fibrillary acaidic protein(GFAP) were detected by immunohistochemistry. RESULTS: hMSC were expanded as undifferentiated cells in culture for more than 15 passages. The isolated cultured MSC comprised a single phenotypic population and displayed a fibroblast-like morphology. These expanded attached MSC were uniformly positive for CD29,CD44,CD90,CD105,CD166 and didn't express CD11a ,CD14,CD34,CD38,CD45,CD80,CD86. FGF-2 have special effect on low denisity MSCs. Simple methods with Tanshinone II A induced hMSC to exhibit a neuronal phenotype, expressing NSE, NF-M, Nestin at 5 hours. But the neuron-like cells didn't express the glial astrocyte marker GFAP. CONCLUSION: hMSC can be induced to differentiate into neurons with Tanshinone II A.
【Key Words】： Bone marrow Mesenchymal stem cells Neurons Tanshinone II A Fibroblast growth factor Cell differentiation
【Fund】： 国家重点研究发展规划项目 ( 973) (No .2 0 0 1CB5 0 990 4 );; 广东省十五专项 (A30 2 0 10 1);; 国家自然科学基金资助项目 ( 30 10 0 188)
【CateGory Index】： R285.5
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 10 Hits
1 FU Yue1, WANG Tong1, LIANG Wei-wen2, FANG Xiang-shao1, HUANG Zi-tong1*1. Department of Emergency, The Second Affiliated Hospital of Sun Yat-sen University, Guangzhou, 5101202. Medical Research Center of Linbaixin;Experimental Study on Isolation, Cultivation and Induced Differentiation of Rat Bone Marrow Stromal Stem Cells[J];Lingnan Journal of Emergency Medicine;2006-01
2 CHEN Xiao-li1,WANG Li-li2,QIAO Yi1,XI Miao-miao2,WU Yin1,Wang Jing-wen1 2 3 1Dept of Pharmacy,Xijing Hospital,The No.4 Military Medical Universit y,Xi'an Shaanxi 710032,China;2Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education,Department of Chemistry,Northwest University,Xi'an Shaanxi 710069,China;Effects of Shuangshen Tongmai Dripping Pills on Brain InfarctionArea and Neuronal Cell Apoptosis of Ischemia-reperfusion in Rats[J];Anti-Infection Pharmacy;2010-03
3 Shi XY,Chen XW,Yuan LJ,Fang Y,Zhou LJ,Hu HM 1Department of Neurology,Anqing Affiliated Hospital of Anhui Medical University,Anqing 246003,Anhui Province,China; 2Department of Neurology,First Affiliated Hospital of Anhui Medical University,Hefei 230022,Anhui Province,China;Effect of total Panax notoginseng saponins on inducing rat bone mesenchymal stem cells differenting into neuron-like cells in vitro:Expression of phenotype and apoptosis protein and survival of neuron-like cells[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2007-42
4 Hu Rong, Xi Huan-jiuAnthropology Laboratory of Liaoning Medical University, Jinzhou 121001, Liaoning Province, China;Bone marrow stroma cells and traditional Chinese medicine to treat cerebral ischemic injury[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2008-43
5 Zeng Xiang-yi1, Wang Wei2, Sun Liang2, Zhang Li2, Zeng Ling-da21Department of Orthopaedics, Second Affiliated Hospital, Liaoning Medical University, Jinzhou 121000, Liaoning Province, China;2Jinzhou Central Hospital, Jinzhou 121000, Liaoning Province, China;Effects of p44/p42 kinase phosphorylation on differentiation of myoblasts into neuron-like cells induced by Salvia miltiorrhiza injection[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2009-19
6 WU Li Mao, LI Lian Da (The Basic Unit,Xiyuan Hospital,China Academy of Taditional Chinese Medicine,Beijing 100091 );Prospect of traditional Chinese materia medica on hepatocyte transplantation[J];Chinese Pharmacological Bulletin;2004-04
7 He Min1),Chen Yunxian1),Zhong Xueyun2)(1)Departement of Hematology,The First Affiliated Hospital of Sun Yat-sen University,Guangzhou 510080;2)Department of Pathology,Medical College of Jinan University,Guangzhou 510630,China);The Plasticity of Bone Morrow Stem Cells and Its Application in Embolismic Diseases[J];Journal of the Graduates Sun Yat-Sen University(Natural Sciences、Medicine);2007-02
8 DENG Yu-bin~1, GUO Xiao-ti~1, LIANG Hai-xiang~2, YUAN Qing-tao~1(Department of Pathophysiology, Zhongshan Medical College, Sun Yat-sen University, Guangzhou 510080 China Department of Orthopaedics ,The Second Hospital of Guangzhou, Guangzhou 510150 China)s;Bone marrow mesenchymal stem cells modified by adenoviral vector containing CTLA4Ig inhibit immune response ex vivo[J];Chinese Journal of Pathophysiology;2004-03
9 WU Gong-xiong1, YANG Zhi-hua2, ZHANG Hai-wei3, LIN Yong-ping4, XU Jie1, ZHANG Chun-xiang2 (1Department of Anatomy, Sun Yat-sen Medical College,?2?Department of Neurology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China;?3?Department of Pathology, Medical College of Jinan University, Guangzhou 510632, China;?4?Department of Verification, Guangzhou Medical College, Guangzhou 510180, China);Therapeutic action of transplantation of marrow mesenchymal stem cells into infarction area after cerebral middle artery occlusion in rats[J];Chinese Journal of Pathophysiology;2004-06
10 ;人胎盘血间质干细胞分离培养[J];Chinese Journal of Pathophysiology;2004-09
【Citations】
Chinese Journal Full-text Database 4 Hits
1 WU Jung-Fang WANG Jie ZHANG Jun-Tian(Institute of Materia Medica, Chinese Academy of Medical Sciences andPeking Union Medical College, Beijing 100050);Protective effects of total salvianolic acids on cerebral hypoxia in mice[J];Chinese Journal of Clinical Pharmacology and Therapeutics;1999-04
2 XIANG Peng 1,XIA Wen-jie 1,ZHANG Li-rong 2,CHEN Zhen-guang 3, ZHANG Xiu-ming 1,LI Yan 1, LI Shu-nong 1 ( 1Department of Pathophysiology, Sun Yat-sen University of Medical Sciences, Guangzhou 510080, China; ? 2?Department of Pathophysiology,;Human mesenchymal stem cells differentiate into neuron-like cells[J];Chinese Journal of Pathophysiology;2001-05
3 XIANG Peng 1,ZHANG Li-rong 2, CHEN Zhen-guang 3, XIA Wen-jie 1, ZHANG Xiu-ming 1,LI Yan 1, LI Shu-nong 1 ( 1Department of Pathophysiology, Sun Yat-sen University of Medical Sciences, Guangzhou 510080, China; ? 2?Department of Pathophysiology;Adult human mesenchymal stem cell differentiates into adipocytes[J];Chinese Journal of Pathophysiology;2001-07
4 ZHANG Li-rong 1, XIANG Peng 2, XIA Wen-jie 2, CHEN Zhen-guang 3, ZHANG Xiu-ming 2, LI Yan 2, LI Shu-nong 2 (? 1?Department of Pathophysiology, Guangdong Pharmacology College, Guangzhou 510224, China; ? 2?Department of Pathophysiology,? 3?Dep;Effects of PD98059 on the differentiation from mesenchymal stem cells to osteoblasts[J];Chinese Journal of Pathophysiology;2002-08
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 FU Wen-yu~(1),WANG Xiao-cui~1,XING Hai-xia~1,LI Feng-jie~2(1.Department of Histology and Embryology,Weifang Medical College,Weifang 261042,China;2.Medical Research Center,Weifang Medical College,Weifang 261042,China);THE EFFECTS OF THE DIFFERENT INDUCERS ON THE DIFFERENTIATION OF RAT BONE-MARROW-DERIVED MESENCHYMAL STEM CELLS TO NEUROCYTES IN VITRO[J];Acta Anatomica Sinica;2007-04
2 WAN Lei-lei,PEI Qing-guo,CAI Xiao-qing,et al. Department of Stomatogy,Shanghai Jiaotong University Affiliated the First People's Hospital,Shanghai 200080;In Vitro Cultivation and Induced Differentiation of Dogs' Bone Marrow Stromal Cells[J];Journal of Oral Science Research;2009-02
3 WU Xao-Mu,YANG Zhi-Gang,DING Wei-Rong,YANG Hai-JUN,LIU Zhe,ZHANG Kun-Nan Institute of Clinical Medicine,The Jiangxi Provincial People's Hospital,Nanchang 330006,China;Differentiation of Human Adult Mesenchymal Stem Cells into Neuron Like Cells in Vitro[J];Chinese Journal of Clinical Neurosciences;2007-04
4 FU Yue1, WANG Tong1, LIANG Wei-wen2, FANG Xiang-shao1, HUANG Zi-tong1*1. Department of Emergency, The Second Affiliated Hospital of Sun Yat-sen University, Guangzhou, 5101202. Medical Research Center of Linbaixin;Experimental Study on Isolation, Cultivation and Induced Differentiation of Rat Bone Marrow Stromal Stem Cells[J];Lingnan Journal of Emergency Medicine;2006-01
5 ZHU Mei-ling,LUO Wei-qiong,ZOU Cui-xian,et al. Shenzhen Baoan Blood Center, Shenzhen 518101;Research on the Human Umbilical Cord Blood Mononuclear Cells Differentiating into Neuron-like Cells in Vitro[J];Journal of Clinical Transfusion and Laboratory Medicine;2007-03
6 Dong Liping, Yuan Fang Beijing Neurosurgical Institute, Beijing 100050, China;The Role of Astrocytes in Neurogenesis[J];Cerebrovascular Diseases Foreign Medical Sciences;2004-01
7 SONG Zhao-feng,JI Xiao-ping,WANG Xiao-rong,JIANG Shi-liang,ZHANG Xiao-fang,WAGN Rong,ZHANG Yun(Department of Cardiology,Qilu Hospital of Shandong University,Key Laboratory of Cardiovascular Remodeling and Function Research,Chinese Ministry of Education and Public Health,Jinan 250012,Shandong,China);Differentiation of cardiomyocytes from bone marrow mesenchymal stem cells in rats in vitro[J];Journal of Shandong University(Health Sciences);2006-10
8 ZHANG Wenzhi,SU Xin,QIN Jinxi. Tianjin Neurosurgical Institute,Tianjin300060,China;Comparison of three methods for directed inducing human bone marrow stromal cells to differentiate into neu-ron-like cells in vitro[J];Modern Journal of Neurology and Neurasurgery;2003-05
9 Wen X,Sun F,Wang JC Department of Neurology,Fifth People's Hospital of Shenyang,Shenyang 110023,Liaoning Province,China;Bone marrow mesenchymal stem cells in the treatment of cerebral infarction[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2007-33
10 Guo Zhen-yu1,Zheng Qin2,Lei Jun-xia 2 1Department of Urinary Surgery,Fifth Hospital of Sun Yat-sen University,Zhuhai 519000,Guangdong Province,China;2Department of Pathophysiology,Zhongshan Medical College,Sun Yat-sen University,Guangzhou 510080,Guangdong Province,China;Comparison of amplification and cell phenotype of murine bone marrow-derived mesenchymal stem cell from different strains[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2008-47
China Proceedings of conference Full-text Database 1 Hits
1 HUA Song, WU Hao, ZHANG Yong (Institute of Blo-Engineering,Northwest Sci-Tech University of Agriculture and Forestry, Yangling,Shaanxi, China ,712100);Advances on Special Differentiation of Bone Marrow Mesenchymal Stem Cells[A];[C];2004
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Zou Fan, Yang Wantong, Tian Jun, et al Department of Rehabilitation,Zhongnan Hospital,Wuhan University,Wuhan 430071,China;The Effect of Angelica Injection on Neuronal Plasticity after Global Cerebral Ischemia[J];Journal of Hubei Medical University;2004-01
2 ZENG Bin, LIN Guosheng, LUO Hao, CAI Jun Dept. of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, China;Study on Inducing Differentiation of Mouse Embryonic Stem Cells into Cardiomyocytes in Vitro[J];Medical Journal of Wuhan University;2006-02
3 Yang Xiaopeng1,Li Qiufang2.1)Department of Neurology,the second affiliated hospital of,Zhengzhou University,Zhengzhou 450014,2)Nusing College,Zhengzhou University,Zhengzhou 450052;Clinical observation on the therapeutic effect of Brevisca Pine in treatment of acute cerebral infarction[J];Chinese Journal of Practical Nervous Diseases;2007-02
4 Guo Jianxin1),Meng Fanchao2).1)Henan Medical College For Continuing Education,Zhengzhou 451191,China 2) Department of Neurology,the Third People's Hospital of Zhengzhou,Zhengzhou 450000,China;Culture and neural differentiation of human adult bone marrow mesenchymal stem cells in vitro[J];Chinese Journal of Practical Nervous Diseases;2009-09
5 HEI Ming-yan, YIN Ping, KUANG Shou-jin(Department of Pediatrics, The Third Xiangya Hospital of Central South University, Changsha 410013, Hunan, China);Effects of Danshentong IIa on the Brain Intracellular Free Calcium in Neonatal Hypoxia-ischemic Rats[J];Journal of Elinical Research;2004-08
6 Ren Xiaoqiao,Li Jiansheng Henan College of Traditional Chinese Medicine;Essential Principle in Treating Senile Ischemic Apoplexy[J];Journal of Henan College of Traditional Chinese Medicine;2003-02
7 Wu Canrong, Tang Xiaomei, He Shuangteng, et al (TCM University of Hunan,Changsha 410007);An Experimental Study on the Oriented Differentiation Inducing Effect of Shenghuang Extract Solution on the Adult Rat Mesenchym Stem Cells into Neuron-Like Cells[J];Journal of Hunan Traditional Chinese Medicine University of Hunan;2003-06
8 YUAN Zhao-kai,CHEN Qing-hua,HUANG Xian-ping,JIAN Wei-xiong,MO Li,HUZhi-xi,SUN Gui-xiang(TCM University of Hunan,Changsha,Hunan 410007,China);Study of compatibility dosis of effective components in Yangxin Tongmai formula on acute myocardial ischemia[J];Journal of Traditional Chinese Medicine University of Hunan;2008-06
9 Fan Yan lei 1,Fan Hong-yu 1,Wang Tian Bao 2 (1.Puyang Hospital of Chinese Me dicine,Puyang,Henan,457000; 2.Xinyang Central Hospital,Xinyang,Henan ,464000);Differentiation and Treatment p lus Qigong Therapy for the Intermediate and Late Stage of 1 53 Cases of Primary Carcinoma of the Liv er[J];HENAN TRADITIONAL CHINESE MEDICINE;2000-03
10 YAN, Jin-Hong ZHANG, Cui-Ping YANG, Pin* (Institute of Molecular Science, Key Laboratory of Chemical Biology of Molecular Engineering of Education Ministry, Shanxi University, Taiyuan 030006);A Study on Inclusion Complexes of Hydroxypropyl-β-cyclodextrin with Tanshinone IIA[J];Acta Chimica Sinica;2006-07
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 ZHOU Jing,JU Lan,YU Ling-fan (Department of ICU,The Second Clinical College,Harbin Medical University,Harbin 150081,China);The effect of mesenchymal stem cells transplantation on sympathetic neural remodeling of the infarcted myocardium[J];Journal of Harbin Medical University;2007-06
2 ZHOU Bo,LIU Feng-yan,ZHENG Yi-qun,ZHANG Er-yong(Liaoning Anshan Fine Chemical Technology and Research Center,Anshan 114051,China);Determination of Tanshinone ⅡA in Ultrasound Extraction of Salvia miltiorrhiza Bge.by HPLC[J];Liaoning Chemical Industry;2008-06
3 ZHU Mei-ling,LUO Wei-qiong,ZOU Cui-xian,et al. Shenzhen Baoan Blood Center, Shenzhen 518101;Research on the Human Umbilical Cord Blood Mononuclear Cells Differentiating into Neuron-like Cells in Vitro[J];Journal of Clinical Transfusion and Laboratory Medicine;2007-03
4 ZHANG Yun-ke1,CHE Zhi-ying2,MEI Xue-rui3(1.Department of Neurology,The First Affiliated Hospital of Henan College of Traditional Chinese Medicine,Zhengzhou 450000,Henan,China;2.Henan University of Traditional Chinese Medicine,Zhengzhou 450008,Henan,China;3.Children's Hospital of Zhengzhou City,Zhengzhou 450000,Henan,China);the Effect of Buyang Huanwu Soup Combined Bone Marrow-derived Mesenchymal Stem Cell Transplantation on the Behavior and Infarct volume to Cerebral Ischemia-reperfusion Rats[J];Liaoning Journal of Traditional Chinese Medicine;2010-02
5 ZHENG Jie, WANG Peixun, ZHOU Lian(Institute of Immunology,Guangzhou University of TCM,Guangzhou 510405,China);Isolation of Rat Marrow Mesenchymal Stem Cells and Effect of Conditioned Medium on Its Proliferation[J];Journal of Guangzhou University of Traditional Chinese Medicine;2006-04
6 SHI Yuan1,SHI Hui2,JIANG Tong-ying1,SUN Chang-shan1,WANG Si-ling1(1.School of Pharmcy,Shenyang Pharmaceutical University,Shenyang 110016,China;2.Shenyang Medical College Fengtian Hospital,Shenyang 110024,China);Preparation and characters studies of tanshinone ⅡA inclusion complex freeze-dried powder injection[J];Journal of Shenyang Pharmaceutical University;2008-10
7 ZHANG Yi-fan~1,ZHU Xiong-xiong~1,ZHU Hui-ping~1,REN Guang-rong~1,MAO Cai-ping~2 (1.Suzhou Municipal Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Traditional Chinese Medicine,Jiangsu Suzhou 215003,China;2.Perinatal Biology Center,Medical College, Soochow University,Jiangsu Suzhou 215123,China);Effects of Sodium TanshinoneⅡA Sulfonate on Hyperlipidemic Fatty Liver Lipid Peroxidation Rats[J];Suzhou University Journal of Medical Science;2009-06
8 Zhu Mei-ling1,Li Yan2,Peng Ai-jun3,Na Xiao-dong2,Lei Jun-xia2,Luo Wei-qiong1,Zou Cui-xian1,Xiang Peng2,Zhang Xiu-ming2,Li Shu-nong2 1Shenzhen Baoan Blood Station,Shenzhen 518101,Guangdong Province,China; 2Department of Pathophysiology,Medical College,Sun Yat-sen University,Guangzhou 510089,Guangdong Province,China; 3Shenzhen Baoan People's Hospital,Shenzhen 518101,Guangdong Province,China;Survival and antigen expression of human cord blood karyocytes in brain of hypoxic-ischemic newborn rats[J];Chinese Journal of Clinical Rehabilitation;2006-37
9 Qiao Chun,Xu Wen-rong Institute of Laboratory Medicine,School of Medical Technology,Jiangsu University,Zhenjiang 212001,Jiangsu Province,China;Gene therapy based on mesenchymal stem cells[J];Chinese Journal of Clinical Rehabilitation;2006-41
10 Wang YC, Zhang CQ, Wang LZ, Wen H, Yin ZM, Wang L, Liu JC, Zhang JY, Yang ZM, Wang JG, Dong YS.1Department of Neurosurgery, the 463 Hospital of Chinese PLA, Shenyang 110042, Liaoning Province, China; 2Department of Athletic Human Science, Shenyang Institute of Physical Education, Shenyang 110032, Liaoning Province, China;Autologous bone marrow-derived mononuclear cell transplant for treatment of nervous system damage and degenerative disease:A report of 42 cases[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2007-20
China Proceedings of conference Full-text Database 1 Hits
1 Qian Jiahua,Fan Minwei,Ma Nengyi (Shanghai Instsute of Chinese Materia Medice);Determinations for Total Tanshinones and Tanshinone Ⅱ_A in Salviae Solid Dispersible Tablets[A];[C];2008
【Secondary Citations】
Chinese Journal Full-text Database 5 Hits
1 Zhang Mingfa ( Dept. of Pharmacology, Shanxi Academy of Traditional Chinese Medicine, Xian 710003);ANTI-ANOXIC EFFECTS OF BERBER1NE IN INTACT MICE[J];Chinese Pharmacological Bulletin;1991-01
2 WANG Jie, WU JunFang, ZHANG JunTian(Institute of Materia Medica, Chinese Academy of Medical Sciencesand Peking Union Medical College, Beijing100050);Studies on the anticerebral ischemia effectof total salvianolic acid[J];CHINESE PHARMACOLOGICAL BULLETIN;1999-02
3 YS Huang;JT Zhang Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing 100050;ANTIOXIDATIVE EFFECT OF THREE WATER-SOLUBLE COMPONENTS ISOLATED FROM SALVIA MILTIORRHIZ A IN VITRO[J];Acta Pharmaceutica Sinica;1992-02
4 GH Du and JT Zhang (Institute of Materia Medica,Peking Union Medical College and Chinese Academy of medical Sciences,Beijing 100050);PROTECTIVE EFFECTS OF SALVIANOLIC ACIDA AGAINST IMPAIRMENT OF MEMORY INDUCED BY CEREBRAL ISCHEMIA-REPERFUSION IN MICE[J];ACTA PHARMACEUTICA SINICA;1995-03
5 XIANG Peng 1,ZHANG Li-rong 2, CHEN Zhen-guang 3, XIA Wen-jie 1, ZHANG Xiu-ming 1,LI Yan 1, LI Shu-nong 1 ( 1Department of Pathophysiology, Sun Yat-sen University of Medical Sciences, Guangzhou 510080, China; ? 2?Department of Pathophysiology;Adult human mesenchymal stem cell differentiates into adipocytes[J];Chinese Journal of Pathophysiology;2001-07
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved