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《Chinese Journal of Pathophysiology》 2003-07
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Mechanism of pulmonary arterial smooth muscle cell apoptosis induced by nitric oxide

TIAN Hong 1, DU Jun-bao 1, FAN Jin 1, ZHAO Wei-hong 2, TANG Chao-shu 3 (? 1?Department of Pediatrics,? 2?Maternal and Child Health Center, ? 3?Institute of Cardiovascular Research, Peking University First Hospital, Beijing 100034, China)  
AIM: To explore the mechanism of apoptosis in pulmonary arterial vascular smooth muscle cells (PASMCs) induced by nitric oxide (NO). METHODS: Wistar rat PASMCs were isolated from explants from the intrapulmonary, incubated with NO donor, sodium nitroprussid (SNP) under 12 or 24 hour normoxic and hypoxic conditions. The cell cycle progression and sub-G 1 of PASMC were analyzed via flow cytometric staining of propidium iodide, and the expression of nuclear transcription factor NF-κB and pro-apoptotic factors caspase-3 were detected using immunochemistry staining. Meanwhile gel electrophoresis of extracted DNA by PASMC was performed. RESULTS: SNP induced PASMC apoptosis in a dose-dependent manner ( P 0.01), which resulted in an increase in apoptotic index by flow cytometric and immuno-expression of caspase-3 on PASMC, and a decrease in immuno-activity of NF-κB on PASMC ( P 0.01) accompanied by DNA laddering. CONCLUSION: Exogenous NO induces rat PASMC apoptosis under normoxia and hypoxia , which appears to be affected by stimulated caspase-3 and NF-κB message pathways.
【Fund】: 北京市自然科学基金项目 ( 70 330 4 7);; 国家重大基础研究发展规划项目 (G2 0 0 0 0 5 6 90 5 );; 国家自然科学基金资助项目 ( 39870 84 4 30 0 70 796 )
【CateGory Index】: R363
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