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《Chinese Journal of Pathophysiology》 2005-03
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Study on inducing differentiation of mouse embryonic stem cells into cardiomyocytes in vitro

WANG Xiu-li 1,2 , WANG Chang-yong 1, YU Xing-ju 2, GUO Xi-min 1, DUAN Cui-mi 1, ZHAO Yun-shan 1, DONG Ling-zhi 1 ( 1Tissue Engineering Research Center, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China;? 2?Institute of Chemical and Physics, Chinese Academy of Science, Dalian 116023, China)  
AIM: To set up a method of inducing mouse embryonic stem cells (mESC) to differentiate into cardiomyocyte after treatment with 5-azacytidine. METHODS: Cytotoxicity of 5-azacytidine was measured by MTT assay. Treatment of mESC with conditioned culture mediums, which were composed of 5-azacytidine alone or combined with retinoic acid, induced the cell differentiation to cardiomyocytes. The cells induced were identified by detecting the expression of cardiac proteins (myosin, desmin, α-actin and α-actinin). Gene MLC-2v, a specific gene of ventricular-like cardiomyocyte, was also detected by RT-PCR. RESULTS: The non-cytotoxic dose of 5-azacytidine was 8 μmol/L, which was able to induce mESC to differentiate into cardiac syncytiums. Cells induced expressed many cardiac proteins and MLC-2v mRNA. However, combined with retinoic acid inhibited mESC differentiation into cardiomyocyte. CONCLUSION: 5-azacytidine is able to promote mESC differentiation into cardiomyocytes. A method of inducing mESC to differentiate into cardiomyocytes in vitro has been established.
【Key Words】： Embryonic stem cells Myocardium Differentiation Azacitidine Tretinoin
【Fund】： 国家“十五”86 3航天领域基金资助项目 (No .2 0 0 2AA74 4 0 6 4 )
【CateGory Index】： R329
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