Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Pathophysiology》 2005-05
Add to Favorite Get Latest Update

The immunity effect of B7-H1 blockade on immature dendritic cells

LI Dong-yin1, MIN Jun~1,2 , CHU Zhong-hua1, WAN Yun-le1, SHAO Jing2, WEI Jing2, LIU Shan-ying2, ZHENG Li-min3, OU Qing-jia1 (1Department of Hepatic-Biliary Surgery,?2?Research Center of Medicine, The Second Affiliated Hospital of Sun Yat-sen University, Guangzhou 510120, Chian;?3?Life Science College, Sun Yat-sen University, Guangzhou 510275, Chain)  
AIM: To investigate the immune stimulation capacity of B7-H1 blockade on immature dendritic cells (DCs) in vitro. METHODS: The human monocyte-derived dendritic cells were induced in the presence of cytokine GM-CSF and IL-4. The expression of B7-H1 was detected by FCM. On blockade of B7-H1, the maturation and endocytic activity, T cells stimulatory proliferation capacity, IL-12 production, T cell differentiation effect of DCs were detected by FCM, MTT assay, ELISA and ELISPOT, respectively. RESULTS: The expression of B7-H1 was increased with the induction of DCs. On day 7, the positive expression was 54.12%, and the TNF-α induced mature DCs had the positive expression rate of 83.64%. The blockade of B7-H1 on immature DCs had sharply increased their T cells stimulatory proliferation capacity and IL-12 production, and efficiently induced the development of Th1/Tc1 cells, but had no effect on their maturation and endocytic activity. CONCLUSION: The blockade of B7-H1 on immature DCs increases its immune stimulation activity. It is valuable to investigate the antitumor immune responses of DCs vaccine with B7-H1 blockade.
【Fund】: 广州市科技攻关重点项目(No.2003Z2-E4011);; 广东省自然科学基金资助项目(No.4009408)
【CateGory Index】: R392
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved