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《Chinese Journal of Pathophysiology》 2006-07
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Effects of electrical stimulation of vagus nerve on gut injury following intestinal ischemia-reperfusion in rats

LIU Chui-liang~1, LI Yu-juan~2, LI Xiang-yu~1, LIU Ke-xuan~3, HUANG Wen-qi~3 (~1Department of Anesthesiology, TCM Hospital of Guangdong Province, ~2 Department of Anesthesiology, The Second Affiliated Hospital of Sun Yat-sen University, Guangzhou 510120, China; ~3 Department of Anesthesiology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China)  
AIM: To investigate the effects of electrical stimulation of vagus nerve on gut injury following intestinal ischemia-reperfusion in rats. METHODS: 30 adult male Wistar rats subjected to bilateral cervical vagotomy were randomly divided into three groups (n=10 per group): (1) Intestinal ischemia-reperfusion group (group I/R): laparotomy and I/R induced by clamping arteria mesenterica superior for 1 h followed by reperfusion for 2 h. (2) Vagus nerve stimulation group (group VNS): laparotomy, I/R and electric stimulation with pulse train of constant amplitude 5V, pulse width 2 ms and frequency 1 Hz at the left caudal vagus ends for 20 minutes before and after occlusion. (3) Sham control group (group SC): sham operation and sham stimulation. Carotid artery was cannulated for mean arterial pressure (MAP) monitoring. A strip of small intestine was taken from distal end of ileum for light microscopic (LM) and transient electron microscopic (TEM) examination at the time of 2 h after reperfusion. Improved Chiu’s scale was used to quantitatively assay the damage degree. The levels of malondialdehyde (MDA) and TNF-α in plasma were detected. RESULTS: MAP in every group kept steady during ischemia, but decreased gradually with the prolongation in the time of reperfusion. MAP decreased more dramaticly in group I/R than that in group VNS (P0.05). Histological changes by LM and TEM were more significant in group I/R than those in group VNS and SC. The improved Chiu’s scale in group VNS was obviously slighter than that in group I/R (P0.05). In group I/R, the levels of MDA and TNF-α in plasma were significantly increased compared with group VNS and SC (P0.01, P0.05). MDA level in group VNS was significantly higher than that in group SC, but there was no significant difference in TNF-α between the two groups. CONCLUSION: Electrical stimulation of vagus nerves can lessen pathological changes of intestinal mucosa induced by I/R and improve BP during reperfusion, which may be related to reducing lipid peroxidation, and down-regulating TNF-α synthesis.
【CateGory Index】: R363
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