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《Chinese Journal of Pathophysiology》 2006-10
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Inhibitory effects of CCK-8 on NF-κB activities stimulated by LPS in rat PIMs

GAO Wei-juan,XU Shun-jiang,CONG Bin,LI Shu-jin,MA Chun-ling,XU Jin-rong,YAO Yu-xia,GU Zhen-yong(Department of Pathophysiology,Hebei Medical University,Shijiazhuang 050017,China.)  
AIM: To investigate the inhibitory effects of cholecystokinin octapeptide(CCK-8) on nuclear factor-κB(NF-κB) activities stimulated by lipopolysaccharide(LPS) by using forskolin,the activator of adenylate cyclase,and PKA inhibitor H-89 in rat pulmonary interstitial macrophages(PIMs).METHODS: PIMs were isolated and purified.EMDA was applied to detect NF-κB activities and Western blotting was used to analyze the IκB-α protein level in rat PIMs.RESULTS: The NF-κB activity was not detected in normal control rat PIMs.The NF-κB activity in LPS-treated rat PIMs was obviously higher than that in control group(P0.01).The IκB-α protein level in endochylema decreased obviously compared to control group(P0.01).No obvious change of NF-κB activity and IκB-α protein level in CCK or Fsk treated rat PIMs was observed(P0.05).The NF-κB activity in CCK+LPS group and LPS+Fsk group were obviously lower than that in LPS group(P0.05).The IκB-α protein level was obviously higher(P0.01).In LPS+CCK+H-89 group,the NF-κB activity in rat PIMs was obviously higher than that in CCK+LPS group(P0.01),while the IκB-α protein level decreased(P0.01).CONCLUSIONS: The activation of cAMP-PKA signaling pathway inhibits the increase in NF-κB activity and the decrease in IκB-α protein level stimulated by LPS in rat PIMs.The anti-inflammatory effects of CCK-8 were taken effect by activating cAMP-PKA signaling pathway and further inhibiting the NF-κB activity.
【Fund】: 国家自然科学基金资助项目(No.30270529);; 河北省自然科学基金资助项目(No.303452);; 河北省普通高等学校博士科研基金资助项目(No.B2003111)
【CateGory Index】: R96
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