Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Pathophysiology》 2006-10
Add to Favorite Get Latest Update

Foam cells can be induced by oxidized low density lipoprotein in human monocyte-derived dendritic cells

LIANG Chun~1,LUO Yu-kun~2,HUANG Dong~2,JIA Qing-zhe~2,XU Cong-feng~2,WANG Ke-qiang~2,WU Zong-gui~1,GE Jun-bo~2(~1Department of Cardiology,Changzheng Hospital,The Second Military Medical University,Shanghai 200003,China;~2 Shanghai Institute of Cardiovascular Disease,Zhongshan Hospital,Fudan University,Shanghai 200032,China)  
AIM: The purpose of this study is to investigate the mechanisms related to oxidized low-density lipoprotein(ox-LDL) and dendritic cells(DCs) in the process of atherosclerosis.METHODS: Human DCs were prepared from human CD14~+ peripheral blood monocytes using rhGM-CSF((100 μg/L)) and rhIL-4(40 μg/L).Cells were incubated with(100 mg/L) native or oxidized LDL for 72 h.The formation of foam cells was investigated by electron microscopy and oil red O staining.Phenotypic and immune functional assays were used with FACS,FITC-dextran phagocytosis,allogeneic mixed T lymphocytes reaction and secretion of Th1/Th2(IL-12/IL-2) cytokines were also conduced.RESULTS: DCs treated with ox-LDL,but not native LDL were induced into foam cells after cultured for 72 h.Compared with native LDL,ox-LDL-treated DCs were less potent in FITC-dextran phagocytosis.ox-LDL promoted allogeneic T cells proliferation.Moreover,ox-LDL upregulated CD80(72.4± 9.6 vs 89.5±10.1,P0.01),CD86(67.2±8.8 vs 80.2±11.6,P0.01), HLA-DR(80.6±9.8 vs 86.6±10.8,P0.01) and CD1a(40.2±10.3 vs 60.2±9.3,(P0.01)) expressions,increased IL-12 secretion [(44.3±8.9)ng/L vs(65.1±10.4)ng/L,P0.05] in DCs.However,the secretion of IL-2 was decreased [(43.6±7.8)ng/L vs(10.0±4.5)ng/L,P0.01] significently.CONCLUSION: DCs were induced into foam cells by ingesting ox-LDL with some functional characteristic of mature DC.DCs seem to be a new source of foam cells and play a key role in immunopathogenesis of atherosclerosis.
【Fund】: 国家重点基础研究发展规划资助项目(No.G2000056903);; 上海市科委资助项目(No.02JC14026)
【CateGory Index】: R363
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 2 Hits
1 LI Xu-sheng, CHEN Guo-rong, HU Ye, JIANG Lei, LI Jian-ming, ZHOU Biao;Study on the gene expression of CD36 in macrophages of diabetic rats[J];Chinese Journal of Pathophysiology;2007-11
2 LIU Yu-de,WANG Song,WU Wei,et al.Department of Cardiology,First Affiliated Hospital of Guangzhou University of Chinese Medicine(Gudngzhou 510405,China);Influence of multiple risk factors on atherosclerosis in mice[J];Chinese Journal of Public Health;2011-09
【Citations】
Chinese Journal Full-text Database 3 Hits
1 LIANG Chun, LUO Yu-Kun, WANG Ke-Qiang, JIA Jian-Guo, HUANG Dong, GE Jun-Bo ZHONG Cui-Ping, SU Min, ZHANG Xin-Hua, GU Yun-Di (Shanghai Institute of Cardiovascular Disease, Zhongshan Hospital, Fudan University, Shanghai 200032);Pattern of vascular dendritic cells distribution in early human aortic atherosclerotic lesions[J];;2003-03
2 QU Zhi-ling, RUAN Qiu-rong, ZHU Da-he (Department of Pathology, School of Basic Medical Science, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China);Native and oxidized low density and very low density lipoprotein enhance the expression of MIP-1α mRNA in aortic smooth muscle cells[J];Chinese Journal of Pathophysiology;2005-03
3 NI Xiao-qing, ZHU Jian-hua, JIANG Sheng-hua;Effects of LDL-containing immune complexes on cholesterol metabolism and excretion of cytokine in monocyte-derived macrophages[J];Chinese Journal of Pathophysiology;2005-11
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LV Yong-heng, LI Zhi-liang. (Department of Cardiology, the Zhujiang Hospital , the Southern Medical University, Guangzhou 510282, China);Significance of dendritic cell in atherosclerosis[J];Journal of Branch Campus of the First Military Medical;2005-01
2 Qu Zhiling,Ruan Qiurong(Department of Pathology, School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China);EFFECT OF NATIVE AND OXIDIZED LOW DENSITY LIPOPROTEIN ON THE EXPRESSION OF VCAM-1 IN HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS[J];Chinese Journal of Histochemistry and Cytochemistry;2007-04
3 LI Chuan-Chang1,3,LIU Wei2,YI Jun1,PU Xiao-Qun1,YANG Tian-Lun1,XIE Qi-Ying1,MO Long1,and CHEN Xiao-Bin1(1.Department of Cardiology,Xiangya Hospital,2.Key Lab of Human Reproduction and Stem Cell Engineering of Ministry of Public Health,Central South University,3.Department of Geriatrics,Xiangya Hospital;Central South University,Changsha,Hunan 410078,China);The Relationship Between Dendritic Cells and Severe Coronary Stenosis and the Effect of Simvastatin Intervention[J];Chinese Journal of Arteriosclerosis;2010-01
4 YIN Cui-Ping,HOU Gui-Hua,FENG Yue-Qiu,KONG Feng,LIANG Ting,and WANG Shu-Mei(Department of Epidemiology and Health Statistics,School of Public Health,Shandong University,Jinan,Shandong 250012,China);Effects of Vaccination with Very Low Density Lipoprotein on Some Immunological and Biochemical Indicators in Neonatal Rats[J];Chinese Journal of Arteriosclerosis;2010-08
5 YIN Xue-liang1,HUI Xue-zhi2,LIU Jian-xiao1,LIANG Tian-shu1,ZHANG Hong2 (1.Zhongshan City Dongfeng Hospital of Kuangdong Province,Guangdong Zhongshan 528425,China;2.The First Affiliated Hospital of Henan University, Henan Kaifeng 475001,China);On effects of simvastatin on human monocyte derived DCs mature and its on immunological functions[J];Journal of Henan University(Medical Science);2007-04
6 SHI Hongyu ZHANG Hongqi SUN Aijun,et al.Shanghai Institute of Cardiovascular Diseases,Zhongshan Hospital,Fudan University,Shanghai 200032;The Distribution of Foam Cells Derived from Dendritic Cells in Atherosclerotic Plaque of Rabbit Model[J];Chinese Journal of Clinical Medicine;2006-02
7 JIA Qing-zhe,YANG Zhi-jian,ZHU Tie-bing,WANG Lian-sheng,LI Chun-jian,WANG Hui,CHEN Bo,MA Wen-zhu,CAO Ke-jiang (Department of Cardiology,the First Affiliated Hospital of NJMU,Nanjing 210029,China);Rosiglitazone inhibits advanced glycosylation end-human serum albumin induced immune maturation of dendritic cells[J];Acta Universitatis Medicinalis Nanjing(Natural Science);2008-06
8 LUO Yu-kun,LIANG Chun △,HUANG Dong,XU Cong-feng,JIA Qing-zhe,WANG Ke-qiang,GE Jun-bo (Department of Cardiology,Zhongshan Hospital,Fudan University-Shanghai Insitute of Cardiovascular Diseases,Shanghai 200032; △Department of Cardiology,Changzheng Hospital, The Second Military Medical University,Shanghai 200032,China);Effects of Oxidized-Low Density Lipoprotein on the Immune Function of Monocyte-Derived Dendritic Cells[J];Journal of Shanghai Medica(University);2004-05
9 LIANG Chun, JIA Qingzhe, LUO Yukun, et al. Department of Cardiology, Changzheng Hospital, Second Military Medical University,Shanghai 200003,China;Pravastatin inhibits immunological function of human monocyte-derived dendritic cells: a new immunomodulatory property of statins[J];Shanghai Medical Journal;2005-04
10 Cao Bao-wei1,Wu Peng2,Qin Xue-mei1,Peng Yi-lan1,Liang Dong-hui3 1Department of Cardiology,Laibin People's Hospital,Laibin 546100,Guangxi Zhuang Autonomous Region,China;2Department of Cardiology,3Department of Traditional Chinese Medicine,Zhujiang Hospital,Southern Medical University,Guangzhou 510280,Guangdong Province,China;Transformation of human peripheral blood monocyte-derived macrophages to foam cells in healthy and coronary heart disease cases[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2008-28
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 CHEN Xiao li 1, CAI Dong lian 1, YANG Qing 2 SONG Li hua 1, LI Ya hui 1,HU Tong jie 1, WEI Li xin 2 (1.Department of Clinical Nutriology, Changhai Hospital, Second military Medical University, Shanghai 200433, China; 2. Tumor Immunology and biotherapy center, Eastern Institute of Hepatobiliary Surgery, Second Military Medical University, Shanghai 200438, China);The study on endothelium damage induced by high dose of methionine[J];Parenteral & Enteral Nutrition;2004-03
2 HE Xiaoyan, ZHANG Min, CHEN Zhichao, YOU Yong, TIAN Lei, ZOU Ping. Department of Hematology, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R.China;Expression of PPAR-γ and its apoptotic significance in lung cancer[J];Chinese Journal of Lung Cancer;2006-01
3 ZHANG Ya-jie YANG Xiao CHEN Wei-ying PENG Wen-xing GUAN Wei-ming LI Xiao-yan YU Xue-qing Department of Nephrology,The First Affiliated Hospital of Sun Yat-sen University,Guangzhou 510080,China;Immunohistologic analysis of renal peroxisome proliferator-activated receptorγexpression in lupus nephritis patients[J];Chinese Journal of Rheumatology;2006-08
4 YU Zhao-xian,CHEN Fei-peng,PAN Qiao-hong.Respiratory Department of The Second Affiliated Hospital of Guangzhou Medical College,Guangzhou 510182,China;Correlations between lung fuction and respiratory impedance by impulse oscillometry in patients with chronic obstructive pulmonary disease(COPD).[J];Guangdong Medical Journal;2007-05
5 FAN Manqi, CHEN Yuying, LIU Ao,et al. Re spir atory Department of Kunming General Hospital of Chengdu Military Command, Kunmin g 650032, China;Respiratory impedance by impulse oscillometry in patients with chronic obstructive lung disease[J];Journal of Practical Diagnosis and Therapy;2005-09
6 LIAO Ming-chu,ZHUANG Ying-zhi,CAO Jian-guo(The First Affiliated Hospital of Nanhua University,Hengyang,Hunan 421001,China);On Functional Mechanism of Combined Use of Rosiglitazone and Cisplatin in Inducing Apoptosis of Hunman Lung Adenocarcinoma Cell[J];Journal of Nanhua University(Medical Edition);2007-01
7 XIA Xue-wei,SU Chang-bao(Department of Neurosurgery,Peking Union Medical College Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100730,China);Anticancer effects of peroxisome proliferator-activated receptor γ agonists[J];Basic Medical Sciences and Clinics;2005-12
8 LIANG Chun, LUO Yu-Kun, WANG Ke-Qiang, JIA Jian-Guo, HUANG Dong, GE Jun-Bo ZHONG Cui-Ping, SU Min, ZHANG Xin-Hua, GU Yun-Di (Shanghai Institute of Cardiovascular Disease, Zhongshan Hospital, Fudan University, Shanghai 200032);Pattern of vascular dendritic cells distribution in early human aortic atherosclerotic lesions[J];;2003-03
9 FAN Bo-Li 1, CHENG Ying 1, FANG Jia-Zhi 1, JIANG Bao-Qi 1, G Neil Thomas 2,Julian AJH Critchley 2,Brian Tomlinson 2 (1.Central Laboratory, Beijing University Shenzhen Hospital, Shenzhen, Guangdong 518036, China; 2.Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Shatin, Hong Kong);Effect of Atorvastatin Treatment on Platelet Aggregation in Whole Blood in the Patients with Hypercholesterolaemia[J];Chinese Journal of Arterial Lerosis;2003-05
10 SHEN Min, LI Lansun. Department of Cardiology, Xijing Hospital, University of 4th Military Medicine, Xi'an 710032, China;Effect of atorvastatin on C-reactive protein and adhesive molecule levels in patients with unstable angina[J];Journal of Clinical Internal Medicine;2003-02
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 LIANG Wei-wei1,YAN Jin2 reviewing,FAN Chun-lei1 checking(1.Teaching and Research Section of Cell Biology,Faculty of Life Science,Zhejiang College of Traditional Chinese Medicine,Hangzhou 310053,Zhejiang,China;2.Teaching and Research Section of Biochemistry,Medical Department,Qianjiang College,Hangzhou Normal University Hangzhou 310012,Zhejiang,China);Active components in Chinese medicine that act on peroxisome proliferator activated receptors[J];Journal of Medical Postgraduates;2010-03
【Secondary Citations】
Chinese Journal Full-text Database 7 Hits
1 Wang Haochuan;Liu Bingwen;Fu Mingde(Institute of Biochemistry and Molecular Biology);Effect of Native and Oxidization-Modified LDL,VLDL and HDL on the Morphological Appearance and Phenotype of Cultured Human Arterial Smooth Muscle Cells[J];JOURNAL OF WEST CHINA UNIVERSITY OF MEDICAL SCIENCES;1995-02
2 Deng Zhongduan, Qu Zhiling Department of Pathology, Tongji Medical University, Wuhan, 430030;A Study On Smooth Muscle Cell—derived Chemotactic Factor[J];Chinese Journal of Arteriosclerosis;1993-01
3 ZHANG Xu Ming, DENG Zhong Duan, QU Zhi Ling,and NI Juan (Department of Pathology,Tongji Medical University, Wuhan 430030, China);Oxidized Low Density Lipoprotein and Very Low Density Lipoprotein Induce the Expression of Macrophage Inflammatory Protein 1α in Human Blood Monocytes[J];Chinese Journal of Arteriosc Lerosis;2001-03
4 QU Zhi Ling, DENG Zhong Duan, and NI Juan (Department of Pathology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China);Native and Oxidized Lipoproteins Induce the Expression of Macrophage Inflammatory Protein 1α in Cultured Human Umbilical Vein Endothelial Cells[J];Chinese Journal of Arteriosc Lerosis;2002-01
5 YAN6 Shi-Lin,DENG Zhong-Duan and QU Zhi-Ling(Department of Pathology,Tongji Medical University,Wuhan 430030, China);Partial Purification of Smooth Muscle Cell-derived Growth Factor[J];CHINESE JOURNAL OF ARTERIOSC LEROSIS;1995-04
6 Wang Chunben;Zong Yiqiang;Wu Wansheng;et al(Department of Biochemistry,School of Basic Medical Sciences,Tongji Medical University,Wuhan 430030);Rapid Isolation of Large Amount of Plasma VLDL and LDL by a Two Step ultracentrifugation[J];ACTAUNIVERSITATIS MEDICTNAE TANGJI;1995-03
7 Wang Guoping,Deng Zhongduan,Qu Zhiling.Department of Patholohgy,Tongji Medical University,Wuhan 430030;Effects of oxidatively modified low density lipoprotein and very low density lipoprotein on the expression of MCP 1 in macrophages[J];NATIONAL MEDICAL JOURNAL OF CHINA;1997-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved