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《Chinese Journal of Pathophysiology》 2007-03
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Antagonistic effect of aminoguanidine on diabitic myocardial damage in rats

LIU Zhao-wei1, SHI Ru-ling1, QI Jin-sheng1, LI Bin1, NIU Tao2 (1Department of Biochemistry, Hebei Medical University, Shijiazhuang 050017, China; 2 Department of Surgery, The Second Hosipital of Shijiazhuang City, Shijiazhuang 050051, China.  
AIM: To investigate the mechanism of the antagonistic effect of aminoguanidine (AG) on diabitic myocardial damage in rats. METHODS: Morphology of myocardium in diabetic group and AG group were observed under transmission electron microscope (TEM). Activity of superoxide dismutase (SOD), nitric oxide synthase (NOS), inducible nitric oxide synthase (iNOS) and contents of malondialdehyde (MDA) were detected biochemically in myocardial homogenate. Nitrotyrosine (NT), which is the sign of peroxynitrite anion (ONOO-), was detected using Western blotting. RESULTS: Under TEM, there was edema around nucleus of cadiocytes, part of inocommaes were breaked and Z lines were ambiguity, and part of costulaes and membranes of mitochondrion in cadiocytes were breaked or disappeared. The activity of NOS, iNOS and the content of MDA and NT increased in diabetic group as compared to control. The pathological changes of myocardium induced by diabetes were reversed in AG group, only part of costulaes of mitochondrion of cadiocytes disappeared, inocommaes were in order on the whole and only some lipid droplets deposited. The activity of NOS, iNOS and the content of MDA and NT in AG group decreased as compared to diabetic group. CONCLUSION: The protective effect of AG on diabitic myocardium may be through anti-lipid peroxidation and decreasing the content of ONOO-.
【Fund】: 河北省卫生厅医学科学重点资助项目(No04062);; 河北省教育厅博士基金资助项目(No132004122);; 石家庄科技攻关项目(No04146173A);; 河北医科大学博士基金资助项目(No040028)
【CateGory Index】: R587.2
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