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Adipose-derived stem cells differentiate to neuron-like cells in vitro

WANG Hong-xiang1,LI Bin-gong2,TANG Xiao-qiong1,ZHAO Zhi-gang1,LI Qiu-bai1,Lü Jian1,ZOU Ping1(1Department of Hematology,2 Department of Cardiology,The Affiliated Union Hospital of Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China.  
AIM: We isolated and purified adipose-derived stem cells(ASCs) from adipose tissue in order to study their characters and examine their neuron differentiation capacity in vitro.METHODS: ASCs were isolated and cultured.The morphology of ASCs was observed under microscope and their phenotype was examined by flow cytometry.All-trans retinoic acid(ATRA) was used to induce ASCs into neuron-like cells.RT-PCR was used to detect the expression of nestin.Immunohistochemistry and Western blotting were used to detect the expression of neurofilament(NF) and neuron specific enolase(NSE),respectively.RESULTS: ASCs displayed a fibroblast-like morphology adhering to the culture plate.The cells expressed several surface antigens such as CD29 and CD105,while did not express CD31,CD34,CD45 and HLA-DR.Under suitable conditions,various passages of ASCs all had the capacity of neuron differentiation.They expressed nestin,NF and NSE 10 days after adding ATRA in the culture system.CONCLUSION: ASCs isolated from adipose tissue can differentiate into neuron-like cells in vitro.ASCs may be used as an alternative source of cells for neural disease therapy.
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