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《Chinese Journal of Pathophysiology》 2007-06
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Expression features of human decorin in E.coli DH5α

YANG Guo-rong1, ZHU Ren-zhi2, HE Xiao-xia3, XUE Yong-jie1, HE Xue-jiao1, YU Rong1(1 Department of Pathology, The First Hospital of PLA, Lanzhou 730030, China; 2 The Institute of Pathology, Lanzhou University, 3 Department of Neurosurgery, People's Hospital of Gansu Province, Lanzhou 730000, China. )  
AIM: To study the expression features of human decorin in E. coli DH5α.METHODS: The pGEX-4T-1-decorin fusion clone was expressed in E. coli DH5α. Positively expressed clone was selected by SDS-PAGE. The optimized inducing time by 1 mmol/L IPTG was determined. The solubility of GST-decorin fusion protein was analyzed by ultrasonic crush method, and its quality was examined by Western blotting. RESULTS: With the induction of 1 mmol/L isopropy-β-D-thiogalactoside (IPTG), the fusion protein was expressed in E. coli DH5α. The optimized inducing time by 1 mmol/L IPTG was 4 hours. Most of fusion protein existed in the form of inclusion body. The expressed protein was GST fusion protein.CONCLUSION: It is suggested that the fusion protein of GST-decorin may be expressed in E.coli DH5α in a large amount in the form of inclusion body.
【Fund】: 甘肃省科技攻关资助项目(No.GK972-2-81A);; 甘肃省自然科学基金资助项目(No.ZS001-A23-058-Y)
【CateGory Index】: R346
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1 ;中医药对TGF-β_1调控作用的研究进展[J];Gansu Medical Journal;2008-02
2 ZHOU Qing1,WANG Dong-ya2,GUO Bing3,ZHANG Guo-zhong3(1.Department of Pathology,Jinggangshan University,Ji'an 343000,China; 2.Department of General Surgeon,Affiliated Hospital of Jinggangshan Univesity,Ji'an 343000,China; 3.Department of Pathophysiology,Guiyang Medical College,Guiyang 550004,China);The dynamic expression and content study of TGF-β1 and smad2/3、4 in the kidneys of diabetic rats[J];Journal of Jinggangshan University;2008-02
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