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《Chinese Journal of Pathophysiology》 2008-04
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Microwave ablation provides effective antigen for induction of anti-tumor immune response

KUANG Ming, LV Ming-de, ZHOU Qi, PENG Bao-gang, LIANG Li-jian, HUANG Jie-fu(Department of Hepatobiliary Surgery, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China. )  
AIM:To investigate the efficacy of microwave ablation (MWA)-coagulated mouse hepatoma tissue in inducing antitumor immune response.METHODS: A model of MWA for s.c. hepatoma in C57L/J mice was established, the anti-tumor effect of MWA-treated mice against Hepa1-6 cells rechallenge and the cytotoxicity of spleen lymphocytes by use of crystal-violet analysis were observed. Mice were immunized with MWA vaccine consisting of MWA-coagulated hepatoma tissue mince, granulocyte-macrophage-colony stimulating factor (GM-CSF) and interleukin-2 (IL-2) slow-release microparticles, the protective anti-tumor immunity induced by MWA vaccine was evaluated.RESULTS: After s.c. challenge of Hepa1-6 cells, hepatoma were observed in 100% mice of control group and 17% of MWA-treated mice. The tumor volumes of tumor-bearing MWA-treated mice were significantly smaller than those in control group, while MWA-treated mice survived longer than control mice. Spleen lymphocytes deprived from tumor-free MWA-treated mice were specific cytotoxic to Hepa1-6 cells. MWA vaccine protected 50% more mice than that using MWA-coagulated tumor tissue alone, and showed similar anti-tumor effect against hepatoma to formalin-fixed tumor vaccine.CONCLUSION: In situ MWA provides effective tumor antigen for induction of tumor-specific antitumor immune response, and the response is amplified by additional use of cytokines.
【Fund】: 国家自然科学基金资助项目(No30672052)
【CateGory Index】: R735.7
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