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《Chinese Journal of Pathophysiology》 2009-05
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Islet cells modified with PD-L1-GPI molecule inhibit attack of reactive T cells

LIANG Ming,LUAN Xiao-jun,CHEN Xiao-yu(Department of Endocrinology,Foshan First Hospital,Foshan 528000,China.)  
AIM:To investigate the immune depressive effect on the reactive T cells and to explore the immunologic injury mechanism of beta cells of islet in type 1 diabetes mellitus(DM-1).METHODS:pAd5/PD-L1-GPI adenovirus vector with target gene was constructed and transfected into NIT cells which are known as a mouse insuloma cell line.The highly expressed membrane protein of PD-L1-GPI was confirmed by Western blotting.The peripheral blood non-adherence lymph leukocytes and target cells were cultured to detect lymph leukocyte proliferation and the T cell function.The level of IL-2,TNF-α and IFN-γ were detected in the cell culture fluid.RESULTS:Compared with the control group,the NIT cells modified with PD-L1-GPI inhibited the sensitized lymph leukocyte proliferation effectively and down-regulated the level of some cytokine secretions such as IL-2,IFN-γ and TNF-α(P0.05).CONCLUSION:The islet cells expressed the PD-L1 gene inhibit the reactive responsive T cells,block up the cytotoxic effect of autoimmunity T cells,and induce the immunotolerance,which would be a value direction of the therapy of DM.
【Fund】: 佛山市科技发展专项资金资助项目(No.200508002);; 广东省医学科技资助项目(No.2004600;No.2007622)
【CateGory Index】: R587.1
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