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《Chinese Journal of Pathophysiology》 2009-09
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Intestinal ischemic postconditioning attenuates acute lung injury induced by intestinal ischemia/reperfusion in rats

LIU Ke-xuan1,LI Yi1,LI Yun-sheng1,LIU Jia-xin1,HUANG Wen-qi1,WU Wei-kang2 1Department of Anesthesiology,The First Affiliated Hospital,2Institute of Integrative traditional Chinese and Western Medicine,Sun Yat-sen University,Guangzhou 510080,China  
AIM: Previous study has shown that brief period of repetitive superior mesenteric artery(SMA) occlusion and reperfusion applied at the onset of reperfusion,ischemic postconditioning(IPo),attenuates intestinal injury after intestinal ischemia/reperfusion(I/R).This study tested the hypothesis that IPo would attenuate intestinal I/R-induced acute lung injury which is comparable to ischemic preconditioning(IPC) and the brief period of postconditioning applied at the onset of reperfusion is critical to pulmonary protection by IPo.METHODS: Rat intestinal I/R injury was produced by clamping SMA for 60 min followed by 60 min of reperfusion.The rats were randomly allocated into one of five groups based upon the intervention(n=8): sham operation(sham): sham surgical preparation including isolation of the SMA without occlusion was performed;injury: there was no intervention either before or after SMA occlusion;ischemia preconditioning(IPC): the SMA was occluded for 10 min followed by 10 min of reperfusion before prolonged occlusion;ischemia postconditioning(IPo): 3 cycles of 30 s reperfusion-30 s reocclusion were imposed immediately upon reperfusion(3 min total intervention);delayed postconditioning(delay): clamping was completely released for full reperfusion for 3 min(the duration of the IPo algorithm),after which 3 cycles of 30 s occlusion and reperfusion were applied.RESULTS: Histological results showed severe damages in rat lungs in the injury group evidenced by increased lung wet/dry weight ratio and pulmonary permeability index,which was accompanied by increases in the levels of plasma TNF-α and IL-6,the pulmonary malondialdehyde(MDA),and the pulmonary myeloperoxidase(MPO) activity,and a decrease in superoxide dismutase(SOD) activity.IPo,not delayed IPo,significantly attenuated lung injury and improved the above variables,which was comparable to IPC.CONCLUSION: IPo at onset of reperfusion reduces acute lung injury induced by intestinal I/R,which may be mediated,in part,by inhibiting oxidant generation,filtration of neutrophils and releases of proinflammatory mediators.The early period of reperfusion in the rat model is critical to pulmonary protection by IPo.IPo may improve outcome in clinical conditions associated with intestinal I/R.
【Fund】: 国家自然科学基金资助项目(No.30672021)
【CateGory Index】: R363
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